摘要
为探讨细菌对消毒剂和抗菌药物耐药性之间的相关性,本试验通过亚抑菌浓度苯扎溴铵和氯已定分别对大肠埃希菌质控菌ATCC25922进行体外诱导培养,测定诱导前后多种抗菌药物最小抑菌浓度(MIC)的变化,并通过实时荧光定量PCR检测诱导前后大肠埃希菌外排泵acrAB-Tolc中融合蛋白AcrA调控基因acrA mRNA的表达量;结果显示,经苯扎溴铵和氯已定诱导后的大肠埃希菌,对恩诺沙星、磺胺二甲嘧啶钠、土霉素、阿莫西林均产生了耐药性,且诱导后的菌株均存在acrA基因mRNA高水平表达,提示大肠埃希菌对消毒剂与抗菌药物之间的耐药性存在相关性,其机制可能是外排耐药机制。
To explore the correlation of resistance between disinfectants and antimicrobial drugs,Escherichia coli control strain ATCC25922 was induced in vitro by subinhibitory concentration of benzalkonium bromide and chlorhexidine,the MIC of various antimicrobial drugs were detected and the expression of acrA which is the regulator gene of fusion protein AcrA of Escherichia coli efflux pumps were tested by real-time fluorescent quantitative PCR before and after induction.The results showed that after induction by benzalkonium bromide and Chlorhexidine,Escherichia coli was resistant to Enrofloxacin,Sulfadimidine sodium,Oxytetracycline and Amoxicillin,and acrA gene was highly expressed.The results indicated that there was correlation between disinfectants and antimicrobial drugs,and the mechanism is probably efflux pump.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2012年第4期615-619,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
重庆市科委自然科学基金项目CSTC(2009BB5303)
重庆市科委科技攻关项目CSTC(2011AB1026)
关键词
苯扎溴铵
氯已定
抗菌药物
耐药性
外排泵
相关性
benzalkonium bromide
chlorhexidine
antibacterial
resistance
efflux pump
correlation
