Survivin在冬凌草甲素介导下对人前列腺癌细胞PC-3凋亡的作用

The role of Survivin in the apoptosis of PC-3 cells induced by Oridonin

目的观察冬凌草甲素对人雄激素非依赖性前列腺癌细胞株-PC-3细胞的诱导凋亡作用，探讨Survivin在此过程中的作用。方法用不同浓度的冬凌草甲素干预PC-3细胞，MIT试验分析观察其对PC-3细胞活力的影响；通过用流式细胞仪分析PC．3早期凋亡细胞的百分率；用Western印迹检法、实时荧光定量PCR方法检测PC-3细胞Survivin的蛋白和mRNA表达的变化。结果（1）细胞生长抑制力呈一定的时间、剂量依赖性，冬凌草甲素浓度为2．5、5、10、20、40μmol／L时，干预48h后相对应的平均细胞生长抑制率依次为9．2％、25．3％、39．3％、77．2％、92．5％，药物抑制PC-3细胞活力的IC50约为10．29μmol／L；流式细胞仪检测经不同浓度的冬凌草甲素（0，10，20，40μmol／L）干预48h后，PC-3细胞的早期凋亡率分别为4．8％，15．4％，19．5％和27．4％（P〈0．05）。（2）冬凌草甲素以浓度依赖性方式抑制PC-3细胞的Survivin的蛋白和mRNA表达。结论冬凌草甲素能以浓度依赖性方式诱导PC-3细胞凋亡。冬凌草甲素通过影响Survivin的表达来诱导PC-3细胞凋亡。

Objective To study the apoptosis-indueing effect of Oridonin on PC-3 cells line and the role of Survivin in the process. Methods After PC-3 cells were incubated with different concentrations of Oridonin, cell viability was analyzed with MTT assay. The percentage of earlier apoptosis cell was ana- lyzed by flow eytometry. The protein expression of Survivin in PC-3 cells were detected by Western blot and fluorescent quantitative PCR. Results Oridonin effectively inhibited the proliferation of PC-3 cells in a concentration-time dependent way. After PC-3 cells were treated with Oridonin （2. 5,5,10,20,40 p, mol/L） for 48 hours,the eytotoxiclty index were 9.2% ,25.3% ,39. 3% ,77.2% ,92. 5% and the ICs0 of PC-3 cells was 10. 29 μmol/L, respectively. Flow cytometry was used to detect the effect of different concentration of Oridonin （0,10,20,40μmoL/L） for 48 hours, the apoptotie rates of PC-3 cells were 4. 8%, 15.4%, 19.5% ,27.4% （ P 〈 0.05 ）. Oridonin down-regulated Survivin protein in a concentration-dependent way in PC-3 cells. Conclusions Oridonin can induce the apoptosis of PC-3 cells by a concentration-dependent manner. Oridonin can induce the apoptosis of PC-3 cells by clown-regulated Survivin protein.