几种齿科合金生物相容性的比较

Compare of Biocompatibility of the Different Dental Alloys

用银钯合金、镍铬合金、钴铬合金、2号合金的浸提液体外培养小鼠成纤维细胞(L929),以含10%胎牛血清的RPMI1640培养基作为阴性对照组。用5种浸提液分别培养L929细胞4 h、7 h、24 h、48 h后,用MTT法检测细胞增值率并计算caspase-3活化度。在荧光显微镜下观察48 h后各组细胞的状态。使用凋亡相关因子caspase-3的表达强度辅以时间梯度分析其与齿科合金生物相容性的相关性。结果表明:加样培养48 h后各实验组均有大量凋亡细胞,但是有明显的不同,在镍铬合金组内可见橙色坏死细胞;各组细胞的毒性为0级,其细胞增值率的排序为:钴铬合金组>2号合金组>银钯合金组>镍铬合金组;各实验组的Caspase-3活性有显著的差异(P<0.01),Caspase-3活化度由高到低的排序为:钴铬合金组>2号合金组银钯合金组>镍铬合金组。各时间点Caspase-3活化度也有显著的差异(P<0.01),其由大到小的排序为:4 h、48 h、7 h、24 h;这几种齿科合金生物相容性由高至低的排序为:钴铬合金>2号合金>银钯合金>镍铬合金。镍离子、银离子是影响其生物相容性的主要因素。在时间梯度的辅助下评价4种齿科合金生物相容性,Caspase-3与MTT的结果一致.

Cultivated L929 cells were fostered by Ag-Pd alloys leaching liquor, Ni-Cr alloys leaching liquor, Co-Cr alloys leaching liquor, self-made 2# alloys leaching liquor （each group sample capacity is 8）. Cultivated Lg2g cells by RPMI 1640 cell medium containing 10% fetal calf serum were taken as the negative control. The cell proliferation rates were detected by MTT, the activity of caspase-3 after 4 h, 7 h, 24 h, 48 h was calculated. The cells condition after 48h was observed by fluorescence microscope. To analyze the relationship between dental alloys biocomoatibilitv and aoootosis correlation factor casnase-3it was assisted by time gradient. The results show that cell quantity of each group are difference, there are lots of apoptosis cells in each group, but obviously different, orange dead cells can be seen in Ni-Cr alloys group after 48h. Cytotoxicity of each group is grade 0, the order of cell proliferation rates of groups is： Co-Cr alloys group〉2# alloys group 〉 Ag-Pd alloys group 〉 Ni-Cr alloys group. The activity of caspase-3 in each group is obviously different （P 〈 0.01）. The order from high to low is： Co-Cr alloys group 〉 2# alloys group 〉 Ag-Pd alloys group 〉 Ni-Cr alloys group. The activity of caspase--3 on the spot of 4 hour, 7 hour, 24 hour and 48 hour is obviously different （P 〈 0.01）. The order from high to low was： 4 hour, 48 hour, 7 hour, 24 hour. The order of these dental alloys biocompatibility from high to low is： Co-Cr alloys group 〉 2# alloys group 〉 Ag-Pd alloys group 〉 Ni-Cr alloys group. Biocompatibility is mainly affected by Ni and Ag ions. Detecting dental alloys biocompatibility by caspase-3 assisted by time gradient, the result is the same as that by MTT.