片段睡眠及其莫达非尼干预对小鼠不同脑区胰高血糖素含量的影响

Effect of sleep fragmentations and pretreatment with modafinil on contents of glucagon in different brain regions of mice

目的研究片段睡眠(SF)对小鼠下丘脑、垂体和枕叶皮质胰高血糖素(Glu)含量的影响及其莫达非尼干预对片段睡眠小鼠上述指标的影响。方法 80只雄性昆明种小鼠随机分为正常对照(C)组,SF模型组,莫达非尼(M)组和SF+莫达非尼(S+M)组。应用自行研制的片段睡眠剥夺平台(专利编号:201807057U),制作SF小鼠模型,SF模型组小鼠和(S+M)组小鼠进行72 h的SF。M组小鼠和(S+M)组小鼠灌胃给予莫达非尼420 mg·kg^(-1)·d^(-1)×3 d。应用放射免疫分析法测定小鼠下丘脑、垂体和枕叶皮质的Glu含量。结果 SF模型小鼠和经过莫达非尼干预的SF小鼠下丘脑、垂体和枕叶皮质Glu的含量显著比C组小鼠增加(P<0.01)。与SF模型小鼠比较,经过莫达非尼干预的SF小鼠下丘脑Glu的含量显著增加(P<0.01),而枕叶皮质Glu的含量显著减少(P<0.01)。结论 SF模型小鼠脑内Glu含量改变,提示中枢Glu可能参与了SF的病理生理过程;经过莫达非尼干预的SF小鼠下丘脑中Glu含量比SF模型小鼠增加,而枕叶皮质中Glu含量比SF模型小鼠减少,提示脑内Glu可能参与了莫达非尼的药理作用。

AIM To investigate the effects of sleep fragmentations （SF） on glucagon （Glu） content in the tissues of hypothalamus, pituitary and occipital cortex in mice and the changes in tissue Glu content of hypothalamus, pituitary and cortex in modafinil-treated SF mice. METHODS Eighty male Kunming mice were randomly divided into normal control （C） group, SF model group, modafinil （M） group, and SF ＋ modafinil （S ＋ M） group. A mouse model of SF was prepared by means of self-developed sleep deprivation experiment equipment （Patent Number： 201807057U）. Seventy-two hours SF was conducted in both SF and S ＋ M mice. Modafinil 420 mg ＂kg-~ ＂d-~ was administered intragastrically for 3 days in both M and S ＋ M groups mice. Radioimmunoassay was used to analyze the tissue Glu content of hypothalamus, pituitary and occipital cortex in mice. RESULTS The contents of Glu were significantly increased in the tissues of hypothalamus, pituitary and occipital cortex of both SF model and modafinil-treated SF mice, compared with control mice （P 〈 0.01）. In comparison with SF mice, the tissue Glu contents were significantly increased （P 〈 0.01 ） in hypothalamus, but significantly decreased （P 〈 0.01 ） in occipital cortex of modafinil-treated SF mice. CONCLUSSION The changes in brain Glu content of the SF mice suggested that the central Glu may be involved in the pathophysilogical processes of the SF; and the increased Glu content of hypothalamus tissue and the decreased Glu content of cortex tissue in modafinil-treated SF mice suggested that the Glu of some brain regions may be involved in the pharmacological effects of modafinil.