脐带间充质干细胞对C57BL/6小鼠Lewis肺癌生长及转移的影响

Effects of umbilical cord-derived mesenchymal stem cells on growth and metastasis of Lewis lung carcinoma in C57BL/6 mice

目的利用Lewis肺癌动物模型探讨脐带来源的间充质干细胞(MSC)对肺癌生长及转移的影响。方法用16只C57BL/6小鼠分别建立Lewis肺癌模型,并随机分为对照组(NS组)和MSC组,每组8只。MSC组分别于接瘤后第7、12、17天时,尾静脉注射脐带MSC(1×106/只),在第21天时处死全部荷瘤小鼠,观察瘤体生长情况及肺转移情况。结果 NS组和MSC组的平均瘤体质量分别为(4.587 5±1.04)g、(4.155±1.13)g,两组相比差异无统计学意义(P=0.59)。NS组和MSC组的平均肺转移数分别为(3.75±1.39)个、(1.13±1.13)个,两组相比差异有统计学意义(P<0.01);抑制转移率为70.0%。结论脐带来源的MSC对C57BL/6小鼠Lewis肺癌本身的生长无影响,但能够抑制肿瘤的转移。

Objective To study the effects of umbilical cord-derived mesenchymal stem cells（MSCs） on the growth and metastasis of lung cancer using Lewis lung carcinoma animal models.Methods Animal models of Lewis lung carcinoma were established in 16 C57BL/6 mice and were randomly divided into normal saline（NS） group and MSC group（n=8）.Mice in the MSC group were injected with 1×10^6 MSCs via the tail vein at day 7,12,and 17.All mice were sacrificed to observe the lung metastases and the tumor size at day 21.Results The average tumor weight was（4.587 5±1.04） g in the NS group and（4.155±1.13） g in the MSC group（P=0.59）.The average number of lung metastasis nodules was 3.75±1.39 in the NS group and 1.13±1.13 in the MSC group（P〈0.01）,showing an inhibitory rate of 70%.Conclusion Umbilical cord-derived MSCs have no effects on the growth of Lewis lung carcinoma in mice,but they can inhibit tumor metastasis.