元胡白芷配方对大鼠外周血中致痛和镇痛物质的影响

Study on the effects of Yuanhuzhitong formula on pain-related substances in formalin-induced pain model and dysmenorrhea rats

目的:研究元胡白芷配方对疼痛大鼠血清中的前列腺素、β-内啡肽和单胺类递质的影响。方法:建立大鼠痛经和福尔马林疼痛模型,采用行为学评价元胡白芷配方的镇痛效果,测定大鼠外周血中的前列腺素类物质、β-内啡肽和单胺类递质的含量。结果:元胡白芷配方药液对两种疼痛模型镇痛效果明显;给药后能降低痛经大鼠血中的前列腺素F2α的含量,降低PGF2α/PGE2(P<0.05);福尔马林模型中,元胡白芷配方可降低外周血中β-内啡肽和5-羟色胺的含量(P<0.05),对多巴胺及去甲肾上腺素无明显影响。结论:元胡白芷配方镇痛效果显著,对外周血中前列腺素、单胺类及β-内啡肽有显著影响,其作用具有多靶点的特点。

OBJECTIVE To investigate the effects of Yuanhuzhitong formula on prostaglandins, β-endorphin and monoamine transmitter of pain models of rats. METHODS Using tool drugs to measure analgesic effect of Yuanhuzhitong formula and detect the content of prostaglandins, β- endorphin and monoamine transmitter in formalin-induced pain model and dysmenorrhea rats. RESULTS Yuanhuzhitong formula had apparent analgesic effect in the experimental models; at the same time, the formula could reduce the content of PGF2oand the ratios of PGF2a and PGE2 （P〈0. 05）in the dysmenorrhea rats. In the formalin-induced pain model, the formula could reduce the content of β- endorphin and 5-HT, while there was no effect on the other monoamine transmitters. CONCLUSION Yuanhuzhitong formula alleviates pain through regulating the content of prostaglandins, β- endorphin and monoamine transmitter of pain models of rats.