氢气对脓毒症小鼠急性肺损伤的影响

Effect of hydrogen on acute lung injury in septic mice

目的评价氢气对脓毒症小鼠急性肺损伤的影响。方法雄性C57BL／6小鼠112只，体重20～25g，5周龄，采用随机数字表法，将其随机分为4组（n=28）：假手术组（A组）、假手术＋氢气组（B组）、脓毒症组（C组）和脓毒症＋氢气组（D组）。采用盲肠结扎穿孔法（CLP）制备脓毒症模型。B组和D组于CLP或假手术后1、6h时分别吸入2％氢气。每组取20只小鼠，记录CLP后7d内生存情况。每组取8只小鼠，于CLP后24h时处死，采集静脉血样，取肺组织，测定血清及肺组织超氧化物歧化酶（SOD）、过氧化氢酶（CAT）及8-异前列腺素F1。（8-iso—PGF2。）水平，测定支气管肺泡灌洗液（BALF）蛋白浓度和肺组织髓过氧化物酶（MPO）活性，进行肺损伤评分，计算肺湿干重比（W／D）。结果与A组比较，C组生存率、血清和肺组织SOD和CAT活性降低，肺损伤评分、W／D、BALF蛋白浓度、肺组织MPO活性、血清和肺组织8-iso—PGF2。水平升高（P〈0．05）；与C组比较，D组生存率、血清和肺组织SOD和CAT活性升高，肺损伤评分、W／D、BALF蛋白浓度、肺组织MPO活性、血清和肺组织8-iso—PGF2。水平降低（P〈0．05）。结论氢气可减轻脓毒症小鼠急性肺损伤，其机制与抑制氧化应激反应有关。

Objective To investigate the effect of hydrogen （H：） on acute lung injury （ALI） in septic mice. Methods One hundred and twelve male C57BL/6 mice, aged 5 weeks, weighing 20-25 g, were randomly divided into 4 groups （ n = 28 each） ： sham operation group （group A）, sham operation ＋ H2 group （group B）, sepsis group （group C） and sepsis ＋ H2 group （group D）. Sepsis was produced by cecum ligation and puncture （CLP） . Groups B and D received 1 h inhalation of 2% H2 at 1 and 6 h after CLP operation or sham operation. Twenty animals in each group were selected and observed for the 7 d survival rate. The left 8 animals in each group were sacrificed at 24 h after CLP operation. Venous blood samples and lung tissues were obtained to determine the levels of superoxide dismutase （SOD）, catalase （CAT） and 8-iso-prostaglandin F2/pha （8-iso-PGF2o） in the serum and lungs, the concentration of protein in bronchoalveolar lavage fluid （BALF）, and the activity of myeloperoxi- dase （MPO） in the lungs. The lung injury score （LIS） was assessed and W/D lung weight ratio was calculated. Results Compared with group A, the 7 d survival rate and activities of SOD and CAT in the serum and lungs were significantly decreased, and LIS, W/D ratio, the concentration of protein in BALF, MPO activity and 8-iso-PGF2a level in the serum and lungs were significantly increased in group C （ P 〈 0.05） . Compared with group C, the 7 d survival rate and activities of SOD and CAT in the serum and lungs were significantly increased, and LIS, W/D ratio, the concentration of protein in BALF, MPO activity and 8-iso-PGF2a level in the serum and lungs were significantly decreased in group C （ P 〈 0.05）. Conclusion H2 can alleviate ALl in septic mice via inhibiting oxidative stress response.