城市回用水灌溉蔬菜的遗传毒性研究

Study on Genotoxic Effect of Vegetables Irrigated with Reclaimed Water

目的研究城市回用水灌溉蔬菜的遗传毒性。方法采用Ames试验(8、40、200、1 000、5 000μg/皿)、小鼠骨髓微核试验(5.0、10.0、20.0 g/kg)和精子畸形试验对城市回用水灌溉的黄瓜和圆白菜的致突变性进行检测。结果Ames试验中试验组和对照组蔬菜(黄瓜和圆白菜)的回变菌落数均未超过空白对照菌落数2倍以上,且不具有剂量-反应关系,试验结果为阴性。小鼠骨髓细胞微核试验和小鼠精子畸形试验均显示各剂量组与对照组比较差异无统计学意义,试验结果均为阴性。结论城市回用水灌溉黄瓜与圆白菜均未显示出具有遗传毒性。

Objective To explorer the genotoxicity of vegetables irrigated with reclaimed water. Methods The vegetables which irrigated with reclaimed water （cucumber and cabbage） were collected as the subject, Ames test （8,40,200,1 000,5 000 μg/plate）, mouse bone marrow micronueleus test and mouse sperm abnormality test （5.0,10.0,20.0 g/kg） were conducted according to the Toxicological Evaluation of Food Safety Procedures and Methods. Results The results of Ames test, mouse bone marrow micronucleus test and mouse sperm abnormality test were all negative. No significant differences were observed in genetic toxicity between the vegetables irrigated with well water and reclaimed water. Conclusion The vegetables irrigated with the reclaimed water did not show genotoxic effect.