凋亡抑制蛋白基因在先天性胆总管囊肿和胆管癌中的表达及意义

Expression of survivin in congenital choledochal cyst and cholanglocarcinoma

目的探讨凋亡抑制蛋白基因（survivin）在先天性胆总管囊肿和胆管癌组织中的表达及意义。为进一步研究胆总管囊肿癌变的机制奠定理论基础。方法采用免疫组化SP法检测Survivin在37例先天性胆总管囊肿组织（其中儿童20例，成人17例）和10例胆管癌组织中的表达，10例正常胆管作为对照。运用SPSS14．0统计学软件处理数据。结果Survivin在37例先天性胆总管囊肿组织中，11例为阴性，15例弱阳性表达，9例阳性表达，2例强阳性表达。而在10例胆管癌组织中，Survivin有5例阳性和5例强阳性表达，未见阴性表达。正常胆管组织中仅有1例弱阳性表达，两两相比差异有统计学意义（P〈0．01）。Survivin在成人胆总管囊肿中的表达强于儿童组，两者间差异有统计学意义（P〈0．01）。结论Survivin参与了胆总管囊肿癌变过程，可能与癌变的发生发展有关，在先天性胆总管囊肿癌变过程中可能起到促进作用。

Objective To investigate the expression of survivin and its significance in congenital choledochal cyst and cholangiocarcinoma. Methods Immunohistochemical （S-P） techique was used to detect survivin in 37 patients with congenital choledochal cyst （children, n = 20, adult, n =7）, and 10 patients with cholangiocareinoma. Ten patients with normal or benign bile duet lesions were used as control. Results In congenital eholedochal cyst, the tissue was strongly positive for 2 patients, positive for 9 patients, weakly positive for 15 patients, and negative for 11 patients. In cholangiocarcinoma, the tissue was strongly positive in 5 patients and positive in 5 patients. In normal bile duct the tissue was weakly positive in 1 patient. The difference among the groups was significant （P〈0.01）. The difference in expression of survivin between adults and children with congenital choledochal cyst was significant. Conclusions Survivin may play a pivotal role in malignant change, tumorigenesis, and tumor progression in congenital choledochal cyst.