Chemerin在肝细胞肝癌中的表达及其预后价值

Chemerin in hepatocelluar carcinoma： expressions and prognostic values

目的探讨Chemerin在肝细胞癌组织及人肝癌细胞株中的表达及预后价值。方法采用免疫组化SABC法检测Chemerin蛋白在正常肝组织、肝癌及癌旁配对组织中的表达情况；采用RT—PCR方法检测肝癌组织、人肝癌细胞株以及永生化肝细胞株LO2中Chemerin mRNA的表达情况。结果Chemerin在肝癌组织中、癌旁配对组织中、正常肝组织中表达阳性率分别为56．67％、90．00％、100％，差别具有统计学意义（P〈0．05）。RT—PCR结果显示，Chemerin mRNA在癌旁配对组织中的表达高于相应的肝癌组织（P〈0．05）。细胞检测结果发现，Chemerin mRNA在永生化的人肝细胞株LO2中高表达；在人肝癌细胞株中低表达。Chemerin蛋白的表达与患者性别、年龄、肿瘤大小、HBsAg状态、甲胎蛋白（AFP）水平无关，而与淋巴结转移、门静脉癌栓、肝癌分化程度以及TNM分期相关。结论Chemerin的表达下调可能在肝癌的发生发展及侵袭转移中起重要作用，提示其可能是影响肝癌患者预后的分子标志。

Objective To study the expressions of Chemerin in hepatocellular carcinoma （HCC） and HCC cell lines, and to demonstrate the relationship between the expressions and prognosis. Methods The expressions of Chemerin protein in normal hepatocellular tissues, HCC and their paired tissues were detected by immunohistochemical staining of SABC; the expressions of Chemerin protein in HCC, cell lines and immortalized hepatocyte cell lines were detected by RT-PCR. Results The positive expression rates of Chemerin were 56.67%, 90.00%, 100% in HCC, HCC cell line and normal hepatocellular tissues respectively, and the differences were significant （P〈0.05）. The expressions of Chemerin mRNA in HCC paired tissues were higher than in HCC （P〈0.05）. There was a higher expression of Chemerin mRNA in immortalized hepatocyte cell line LO2 ; but a lower expression in HCC cell lines. The expressions of Chemerin were related to lymph node metastasis, portal vein tumour thrombi, differentiation and TNM stage hut not related to sex, age, tumour size, HBsAg and AFP. Conclusions Down-regulated expression of Chemerin may play an important role in the development, progression and metastasis of HCC. It may be a molecular marker for prognosis of HCC.