活化的肝星状细胞促进调节性T细胞的表达

Activated hepatic steilate cells promote expression of regulatory T cells

目的越来越多的研究证实，活化的肝星状细胞（hepatic stellate cells，HSCs）具有免疫抑制功能。本研究观察了HSCs对T细胞介导的适应性免疫应答的影响及其与调节性T细胞（regulatory Tcells，Treg）的关系。方法分离培养小鼠HSCs，运用混合淋巴细胞反应（mixed lymphocyte reaction，MLR），体外观察活化的HSCs对T细胞增殖和Treg细胞的影响。结果活化的HSCs能导致树突状细胞（dendritic cells，DCs）介导的适应性免疫应答中T细胞的低反应性：抑制T细胞的增殖，同时诱导Treg细胞的表达。结论活化的HSCs能通过促进Treg细胞表达而诱导适应性免疫应答中T细胞的活化无能，促进免疫耐受。

Objective Accumulating reports have suggested that hepatic stellate ceils （HbCs） exhibit immunosuppressive ability and may he responsible for the occurrence and development of hepatocellular carcinoma （HCC）. The mechanisms through which HSCs affect T-cell-induced adaptive im mune responses and the relationship with the regulatory T cells （Treg cells） were studied. Methods We isolated HSCs from wildtype mice to demonstrate the influence of HSCs on T-cell proliferation and explored their effect on Treg cells through mixed leukocyte reactions （MLRs） in vitro. Results We found that activated HSCs could induce T-cell hyporesponsiveness in adaptive immune response by inhibiting the proliferation of T ceils andinereasing the quantity of Treg ceils. Conclusion Activated HSCs may lead to hypoergia of T cells in adaptive immune reaction and up-regulate the expression of Treg cells, thus facilitating immunotolarance.