利用薯蓣皂素生产废水发酵生产谷胱甘肽

Study on Using Wastewater of Dioscorea zingiberensis C.H. Wright to Produce Glutathione by Fermentation

经过传统工艺酸解盾叶薯蓣得到的皂素生产废水是一类酸性高浓度有机废水,实验主要通过对皂素废水进行了3种不同方式的预处理,实现废水发酵生产谷胱甘肽,同时降低废水COD值的目的。实验表明,3种不同的预处理方式对谷胱甘肽产量影响不同,将纯糖液的谷胱甘肽产率记作100%,处理1,只调节废水pH值,发酵得到的谷胱甘肽产量为0.847 625 g/L,达到纯糖液发酵的54.79%,同时废水COD去除率达57.17%;处理2,先调节废水pH值,然后进行活性炭吸附之后发酵,得到谷胱甘肽产量为0.835 7 g/L,达到纯糖液发酵的55.02%,同时废水COD去除率达77.60%;处理3,先进行活性炭吸附,然后调节其pH之后发酵,得到的谷胱甘肽的产量为0.689 0 g/L,达到纯糖液发酵的44.55%,同时废水COD的去除率达到73.52%。从工业生产成本的角度出发,处理2的方式为最佳方式。

The wastewater of Dioscorea zingiberensis C.H.Wright using the traditional hydrolyze process technology is a kind of organism waste that is high in concentration and acidity.In this study,pretreated with the wastewater was pre-treated by Dioscorea zingiberensis C.H.Wright in three different ways,and the goal of producing Glutathione by fermentation meanwhile reducing the COD value of wastewater was achieved.The result indicated that the three different pretreatment methods influenced Glutathione yield.Given the definition of Glutathione yield ratio fermented in pure sugar liquid 100%,process 1： by only adjusting pH,the fermentation yield of Glutatione reached 0.847 625 g/L,accounting for 54.79 % of the pure sugar fermentation medium,and the removal rate of COD was 57.17 %;process 2： by adjusting pH first and then absorbed with activated carbon,the fermentation yield of Glutathione reached 0.835 7 g/L,accounting for 55.02 % of the pure sugar fermentation medium,and removal rate of COD was 77.60 %;process 3： absorbed with activated carbon first and then adjusted pH,the fermentation yield of Glutathione reached 0.689 0 g/L,accounting for 44.55 % of the pure sugar fermentation medium and removal rate of COD was 73.52 %.Taking production cost in consideration,process 2 is the best.This study has achieved good environmental and economical benefits and provided theoretical support for industrial production by recycling the valuable resources of wastewater.At present report on such study is not available at home and abroad.