雪荔组方挥发油成分分析及体外抑菌活性研究

Chemical Constituents and Anti-bacterial Activity of Essential Oil from Compound Xueli

目的研究雪荔组方中挥发油的化学成分及其抗菌活性。方法采用水蒸气蒸馏法提取雪荔组方中的挥发油,用GC-MS进行成分分析,面积归一化法测定各成分的质量分数;并采用微量肉汤稀释法测定挥发油的抑菌活性。结果水蒸气蒸馏法提取的挥发油共鉴定出43种成分,占挥发油总成分的85.21?,其中主要含棕榈酸(30.46?),匙桉醇(15.70?),1,2,3,4,4a,7-六氢-1,6-二甲基-4-异丙基萘(6.71?),氧化石竹烯(6.13?),2,6-二叔丁基对甲酚(5.93?)等。挥发油在体外对金黄色葡萄球菌、白色念珠菌、绿脓杆菌、大肠杆菌、奇异变形杆菌均有一定的抑菌作用。结论雪荔组方挥发油具有多种活性成分,有一定的抑菌活性,为进一步合理开发组方提供了科学依据。

OBJECTIVE To study the chemical constituents and anti-bacterial activity of essential oil from compound Xueli.METHODS The essential oil was extracted from compound Xueli by steam distillation.The constituents from the essential oil were analyzed by GC-MS and the amount of the constituents was calculated by normalization method.The anti-bacterial tests of MIC and MBC for the essential oil were studied by using broth micro-dilution method.RESULTS Forty-three constituents were separated and identified,accounting for 85.21 of the total essential oil.The main constituents were palmitic acid（30.46）,1H-cycloprop[e]-azulen-7-ol（15.70）,naphthalene,1,2,3,4,4a,7-hexahydro-1,6-dimethyl-4-（1-methylethyl）（6.71）,caryohyllene oxide（6.13）,and 2,6-di-tert-butyl-p-cresol（5.93）.The essential oil showed some certain anti-bacterial activities against the tested strains including Staphylococcus aureus,Blastomyces albicans,Pseudomonas aeruginosa,Escherichia coli,and Proteus hauseri.CONCLUSION The essential oil of compound Xueli contained various active constituents which showed certain anti-bacterial activity.The study will provide some scientific references for the further research of compound Xueli.