荜茇提取物对地鼠脂质代谢紊乱的影响

Effects of Extracts of Piper Longum on the Disorder of Lipid Metabolism in Hamsters

目的研究荜茇提取物对高血脂症地鼠血脂的影响。方法给予高脂饲料建立金黄地鼠高血脂模型,将动物随机分为正常对照组、模型组、辛伐他汀组、荜茇提取物组、去胡椒碱荜茇提取物组,除正常对照组外其余各组均给予高脂饲料喂养2或4周,每周测量体质量,分别在第2周末和第4周末用全自动生化分析仪检测血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、肝脏指数、肝脂质TC和TG。结果与正常对照组比较,模型组TC,TG和LDL显著增加(P<0.01),HDL显著降低;与模型组比较,荜茇提取物组在第2周和第4周均能显著降低TC,TG和LDL,显著升高HDL(P<0.05或P<0.01);去胡椒碱荜茇提取物组在第2周能显著降低LDL,升高HDL,在第4周有降低TC,TG,LDL的趋势,但差异无统计学意义,能显著升高HDL(P<0.05);各受试物组动物在前两周体质量显著低于模型组,而后2周差异无统计学意义;4周时荜茇提取物组能显著降低肝脂质TC,TG;各受试物组均能显著降低肝指数。结论荜茇提取物可显著改善高血脂症地鼠血脂水平,其中胡椒碱为其主要有效成分,且与其他各成分有协同降血脂作用。

OBJECTIVE To explore the effects of extracts of Piper longum on golden hamster models of hyperlipidemia.METHODS The disorder of lipid metabolism was induced by feeding with high-cholesterol diets.The hamsters were randomly divided into normal control group,hyperlipidemia model group,extracts of Piper longum group,removal of piperine group and sinvastatin positive control group.The later 4 groups of hamsters were fed with high-cholesterol diets in addition to the normal control for 2 or 4 weeks.The weights of all groups were measured.Total cholesterol（TC）,triglycerides（TG） concentrations in the serum and the liver and low density lipoprotein cholesterol（LDL-C）,high density lipoprotein cholesterol（HDL-C） in the serum concentrations were detected by auto-biochemistry analyzer.The liver indexes were determined.RESULTS Compared with normal control,model group significantly increased TC,TG,LDL-C（P0.01） and obviously decreased HDL-C.Compared with model group,extracts of Piper longum group could significantly reduce TC,TG,LDL-C and significantly increase HDL-C in the end of 2 weeks and 4 weeks（P0.05 or P0.01）.Except removal of piperine group could notably decrease LDL-C and increase HDL-C in the end of 2 weeks,the contents of TC,TG,LDL-C of removal of piperine group were lower than the model group in the other time,but there were no significant differences,and the HDL were higher than the model group（P0.05）.In the first two weeks,compared with the model group,the weight of tested groups significantly higher,but there was no significant difference after two weeks.Extracts of Piper longum group could obviously decrease the contents of liver lipid.The tested groups could notably decrease liver index.CONCLUSION The extracts of Piper longum could adjust the disorder of lipid metabolism in hamster models of hyperlipidemia,and piperine is the main active ingredient,moreover,there is a synergistic effect between piperine and other components in extracts of Piper longum.