HPLC测定六味地黄浓缩丸中5-HMF的含量

Determination of 5-Hydroxymethylfurfural in Liuweidihuang Pills by HPLC

目的建立六味地黄浓缩丸中5-羟甲基糠醛（5-HMF）的含量测定方法。方法采用Agilent Zorbax C18色谱柱（250 mm×4.6 mm,5μm）;流动相为甲醇-水（20∶8 0）;流速：1.0 mL.min？1;检测波长：280 nm;柱温：30℃。结果 5-HMF在0.026 2~2.62 mg.mL？1内线性关系良好,平均回收率为99.7%,RSD为0.57%。结论该方法简便、快速、准确度好,可有效控制该制剂中5-羟甲基糠醛含量。

OBJECTIVE To establish an HPLC method for the determination of 5-hydroxymethylfurfural（5-HMF） in Liuweidihuang pills.METHODS The HPLC method was performed on an Agilent Zorbax C18 column（250 mm×4.6 mm,5 μm） with 284 nm as the detection wavelength,and methanol-water（20∶80） was used as mobile phase.The column temperature was 30 ℃,and the flow rate was 1.0 mL·min 1.RESULTS The regression results which was based on weighted least square method showed a good linearity within the range of 0.026 2 2.62 mg·mL 1,and the average recovery was 99.7% with RSD of 0.57%.CONCLUSION The HPLC method was accurate,sensitive and suitable for determining the content of 5-HMF in Liuweidihuang pills.