摘要
目的了解医院耐甲氧西林金黄色葡萄球菌(MRSA)的SCCmec基因型别及耐药特征。方法应用聚合酶链反应(PCR)技术检测MRSA菌株的mecA基因并对其进行分型,应用E-test法测试MRSA对万古霉素、替考拉宁、利奈唑胺、左氧氟沙星和利福平5种药物的MIC值。结果 62株MRSA的mecA基因全部阳性,SCCmecⅢ型60株,SCCmecⅣa型1株,还有1株用本实验方法未能分型。药敏试验显示所检菌株对万古霉素、替考拉宁和利奈唑胺均敏感,对左氧氟沙星和利福平的耐药率分别为96.8%和82.3%。结论该院以SCCmecⅢ型为主要流行类型,治疗MRSA仍首选万古霉素等糖肽类抗菌药物。
Objective To investigate antimicrobial resistance and Staphylococcal cassette chromosome mec（SCCmec） genotype characteristics of methicillin-resistant Staphylococcus aureus（MRSA） in this hospital.Methods Antimicrobial susceptibility of MRSA was detected by E-test method,and polymerase chain reaction（PCR） were used to determine the genotype of SCCmec.Results All of 62 strains of MRSA isolate were mecA positive,with 1 isolate of SCCmecⅣa,60 isolates of SCCmecⅢ and 1 isolate with unidentified genotype.All isolates were susceptible to Vancomycin,Teicoplanin and Linezolid,and the resistance rate of the 62 isolates to Levofloxacin and Rifampicin were 96.8% and 82.3% respectively.Conclusion SCCmecⅢ genotype might be the major epidemic isolate in this hospital and Vancomycin could be the first choice to treat MRSA infection.
出处
《国际检验医学杂志》
CAS
2012年第7期769-770,773,共3页
International Journal of Laboratory Medicine
基金
陕西省科学技术研究发展计划项目(项目编号2010K16-01-07)
