50Hz磁场慢性暴露对雄性大鼠生殖系统和外周血细胞的影响

Effects of chronic exposure to 50 Hz extremely low frequency electromagnetic field(ELF-EMF) on reproductive system and peripheral blood cells in rats

目的研究50Hz磁场慢性暴露对雄性大鼠生殖系统和外周血细胞的影响。方法将SD大鼠随机分为1个对照组和3个辐照组,分别全身暴露于0(对照组)、20、100及500μT 50Hz磁场中,每天2h,连续4周,每周测其体质量变化,暴露结束后收集外周血和睾丸组织,检测大鼠血清睾酮水平和外周血细胞,测定睾丸脏器系数,光镜病理学分析大鼠睾丸组织形态结构。结果暴露期间各组大鼠体质量无明显差异。20μT和100μT剂量的暴露对血清睾酮水平、睾丸脏器系数、外周血细胞均无明显影响。500μT剂量暴露后大鼠血清睾酮水平较对照组差异有统计学意义(P<0.05),睾丸脏器系数差异无统计学意义(P>0.05),外周血白细胞数量较对照组显著增加,差异有统计学意义(P<0.05)。HE染色发现,大鼠睾丸间质组织可见局灶性充血水肿现象,并随着暴露剂量的增加,充血水肿越明显,呈剂量-效应关系。结论在该实验条件下,50Hz(500μT)磁场慢性暴露对雄性大鼠生长发育无明显影响,可引起大鼠睾丸结构和功能的变化以及外周血白细胞数量的增加。

Objective To evaluate the effects of chronic exposure to 50 Hz extremely low frequency electromagnetic field（ELF-EMF） on reproductive system and peripheral blood cells in rats.Methods SD rats were randomly divided into 4 groups and exposed to ELF-EMF with 0（control group）,20,100 and 500 μT for 2 hours every day.After 4 weeks,blood samples were collected for detecting serum testosterone and peripheral blood cell counts,testes were removed for weighting and histological analysis.Results The number of white blood cell and serum testosterone level were significantly increased in 500 μT group compared with control group（P0.05）.Moreover,there was focal hyperemia and edema in the interstitial tissue of the exposed testes.Other significant difference was not found.Conclusion Chronic exposure to ELF-EMF with 500 μT might not affect the growth and development of male rats,but adversely affect reproductive and hematological system.