中药胃炎消治疗大鼠慢性萎缩性胃炎的病理研究

Pathologic studies of Chinese drug Weiyanxiao on experimental chronic atrophic gastritis in rats

目的 观察中药胃炎消对实验性大鼠慢性萎缩性胃炎的治疗作用。 方法 采用主动免疫、去氧胆酸钠、热水灌胃综合法连续造模90d,复制慢性萎缩性胃炎(CAG)大鼠模型,胃炎消按照临床用量的5倍、10倍(12.3g/kg,24.6g/kg)灌胃给药,连续治疗60d,进行胃液分析及病理组织学观察,光镜下观察胃粘膜的炎症程度并分级计分,用测微尺测量胃粘膜厚度、粘膜肌层厚度、胃体部胃小凹深度及胃腺长度并取其比值。 结果 非治疗模型组大鼠胃窦部、窦体交界、前胃与腺胃交界处的炎症计分分别为1.98±0.16,1.53±0.51,1.81±0.40,明显高于正常对照:1.54±0.49(P<0.01),1.20±0.14(P<0.05),1.40±0.54(P<0.05);胃窦部粘膜厚度(μm)为278±32,明显少于正常对照349±33(P<0.05);胃窦部、胃体部粘膜肌层厚度(μm)分别为46±8,57±15,比正常对照明显增厚(32±6,P<0.05,29.6±16,P<0.01);胃小凹与胃腺体长度比值(0.34±0.05)明显高于正常对照(0.26±0.02,P<0.05),治疗组大鼠经胃炎消治疗60d,其胃粘膜炎症程度较非治疗组减轻,以胃窦部炎症改善明显,小、大剂量组分别为1.75±0.23,1.68±0.20,比非治疗组明显降低(1.98±0.16,P<0.05);同时胃窦部粘膜厚度增加,以大剂量组作用明显,为329±32,明显高于非治疗组(278±32,P<0.01);大剂量组胃窦部?

AIM To study the effects of Weiyanxiao (WYX) on experimental chronic atrophic gastritis(CAG) in rats. METHODS CAG model in rats was made using complex methods of active immunization combined with oral administration of sodium deoxycholate and hot water for 90 d, followed by the treatment with administration of WYX orally in the dosages of 12.3 g/kg and 24.6 g/kg (5 and 10 times according to the clinic dosages) for 60 d. Then the acidity of gastric juice was measured using acid-base balance. The histopathological changes including the depth of mucosa, muscular layers, gastric pit and length of gastric glands were measured with micrometer under optical microscope. The degrees of inflammation in gastric mucosa were evaluated with score according to the numbers of inflammatory cells. RESULTS In untreated group, the inflammatory score of gastric antrum, antrum-body junction and proventriculus-body junction were 1.98 μm±0.16 μm, 1.53 μm±0.51 μm and 1.81 μm±0.40 μm respectively, which were significantly higher than that of normal group (1.54 μm± 0.49 μm, P<0.01, 1.20 μm±0.14 μm, P<0.05, 1.40 μm± 0.54 μm, P< 0.05); mucosa depth in antrum was 278 μm± 32 μm, which is significantly lower than that in normal group (349 μm±33 μm, P<0.05); the depth of mucosa muscular layers of gastric antrum and body were 46 μm± 8 μm, 57 μm±15 Mm which were significantly increased compared to that of normal group (32 μm±6 μm, P<0.05, 29.6 μm±16 μm, P<0.01); the length ratio of gastric pit vs gland (0.34±0.05) increased significantly compared to that in normal group (0.26±0.02, P<0.05). After WYX treatment for 60 d, the gastric mucosa inflammation was improved, especially in antrum (large dosage 1.75±0.23, small dosage 1.68±0.20), which were significantly decreased compared to untreated rats (1.98±0.16, P<0.05); mucosa length in antrum increased, especially in the large dosage group (329 μm±22 μm) which was significantly higher than that in untreated group (278 μm±32 μm, P<0.01 ). In large dosage group, mucosa muscular layers of antrum (36 μm±6 μm) and body (44 μm±11 μm) were became thinner than that in untreated group (46 μm±8 μm, P<0.05, 57 μm±15 μm, P<0.05); the ratio of gastric pit vs gland was decreased as compared with untreated group; acidity of gastric juice was recovered to normal level.CONCLUSION WYX has significant effects in treating experimental CAG.