摘要
目的 研究ＤＴ３９０基因与ＶＥＧＦ人外显子７基因晤后对胃癌细胞的杀伤作用，探索毒素晤基因治疗胃癌的有效途径，方法 构建携带ＤＴ９０－ＶＥＧＦ１６５或ＤＴ３９０－ＶＥＧＦｅｘｏｎ７融合基因的真核表达载体，用脂质体介导转染胃癌细胞ＳＧＣ７９０１，观察胃癌细胞的形态变化，结果 ５μｇ趑 核表达构的转染５×１０＾５个胃癌细胞／孔（２４孔板）９６ｈ后发现，转素融合基因的细胞９０％死亡，而对照细胞形态正常。
AIM To study the killing effect of the fusion geneconsisting of DT390-VEGF165 or DT390-VEGFexon7 on humangastric carcinoma cell.METHODS The eukaryotic expression vector carryingDT390-VEGF165 or DT390-VEGFexon7 gene was constructed.The eukaryotic expression constructs were introducedinto gastric carcinoma cell SGC7901 by means oflipofectamine-mediated gene transfer procedure. Thecytolytic effect of the chimeric gene was registered byphotomicrography.RESULTS The most gastric carcinoma cells of 5×105cells/ well were cytosis after transferred with 5 μgeukaryotic expression constructs 96 hours later, but thecontrol cells were normal.CONCLUSION The chimeric gene can be expressed in thegastric carcinoma cell and result in obvious antitumoreffects.
出处
《世界华人消化杂志》
CAS
2000年第4期393-396,共4页
World Chinese Journal of Digestology
基金
国家自然科学基金
No.39//0834
关键词
真核表达载体
胃肿瘤
基因治疗
VEGF
diphtheria toxin gene
vascular endothelial growth factor gene
eukaryotic expression vector
stomach neoplasms
gene therapy
