基于rbcL序列鉴别十大功劳叶及其伪品

Molecular identification of Folium Mahonia and its adulterants using rbcL sequence

目的:利用叶绿体基因rbcL序列探讨十大功劳叶及其伪品枸骨叶的分子鉴定方法。方法:分别提取十大功劳叶的植物基因组DNA,经PCR扩增后测序,利用相关软件对序列进行分析。结果:所得各样品的rbcL序列长度为655bp,共存在碱基差异49bp,从构建的系统分枝树可知,各物种可明显分开。结论:rbcL序列可用于鉴定十大功劳叶及其伪品。

Objective：To perform the molecular identification for Folium Mahonia and its adulterants using rbcL gene sequences.Methods： The DNA of Folium Mahonia was extracted and gene sequence was amplified by PCR.PCR product was directly sequenced after purification.Sequences were analyzed with related software.Results：The rbcL gene length of the proceeds of each sample was 655bp.The number od variation sites is 49bp.In the cluster dendrogram,all species distinguished from each other.Conclusion ：The rbcL gene could be used to identificate Folium Mahonia and its adulterants.