HPLC法测定桂丹莪棱口服液中芍药苷的含量

Contents determination of Paeoniflori in Guidan′eleng Mixture by HPLC

目的建立桂丹莪棱口服液的质量标准。方法采用HPLC法对桂丹莪棱口服液中芍药苷的含量进行测定。结果桂丹莪棱口服液中芍药苷的含量在0.308 2～6.164 0μg的范围内,有良好的线性关系,r=0.999 9,平均回收率为98.68%,RSD=1.01%,桂丹莪棱口服液中芍药苷的含量为1.392 mg/mL。结论建立了桂丹莪棱口服液的质量标准,该方法操作易行,稳定可靠,可用于桂丹莪棱口服液中芍药苷的含量及该制剂的质量控制。

Objective To establish the standard for determination of Paeoniflori in Guidan′eleng Mixture.Methods Paeoniflori in Guidan′eleng Mixture were determined by HPLC.Results The linear range of Paeoniflori was 0.308 2-6.164 0 μg,r = 0.999 9.The average recovery of Paeoniflori was 98.68% and RSD was 1.01%.Contents determination of Paeoniflori in Guidan′eleng Mixture was 1.392 mg/mL.Conclusion The method is simple,sensitive and accurte,and can be used to determine Paeoniflori and as the quality control of Guidan′eleng Mixture.