杭州市家蝇对常见杀虫剂的抗药性调查分析

Survey on the resistance of Musca domestica to insecticides in Hangzhou city

目的了解杭州市家蝇抗药性水平,为杀虫剂的合理使用提供科学依据。方法采用微量点滴法检测杭州市家蝇现场种群对溴氰菊酯、高效氯氰菊酯、二氯苯醚菊酯、EBT、敌敌畏、胺菊酯6种杀虫剂抗药性水平。结果6种杀虫剂对家蝇现场种群的半数致死剂量(LD50),其化学毒性强度由低到高依次为二氯苯醚菊酯、溴氰菊酯、胺菊酯、EBT、高效氯氰菊酯、敌敌畏。与敏感品系比较,溴氰菊酯抗性水平提高最多,抗性系数为338.00(LD50为0.1014μg/只);其次为高效氯氰菊酯,抗性系数为86.77(LD50为0.7983μg/只);其他杀虫剂抗性水平接近或低于敏感品系,抗性系数在0.19～1.92。结论杭州市家蝇现场种群对溴氰菊酯、高效氯氰菊酯的抗性水平较高,敌敌畏等抗性呈升高趋势,在灭蝇工作中应注意避免大量单独使用。

Objective To investigate the resistance of Musca domestica to insecticides for providing scientific information for an effective use of insecticides.Methods The resistance of field strains of M.domestica to deltamethrin,beta-cypermethrin,permethrin,esbiothrin,DDVP and tetramethrin was determined by topical application methods.Results The chemical toxicity of the six insecticides in terms of LD50 ascended in the order of permethrin,deltamethrin,tetramethrin,EBT,beta-cypermethrin and DDVP.Compared with the susceptible strain,the resistance to deltamethrin increased the most with a resistance coefficient of 338.00（LD50=0.1014 μg per piece）,followed by cypermethrin which had a resistance coefficient of 86.77（LD50=0.7983 μg per piece）.The coefficients of the resistance to the rest insecticides were close to or lower than that of the susceptible strain anywhere from 0.19 to 1.92.Conclusion The field strains of M.domestica in Hangzhou has a relatively high resistance to deltamethrin and cypermethrin.The resistance of M.domestica to DDVP takes on an increasing tendency.So frequent single use of it should be avoided in future control of M.domestica.