摘要
目的建立咽炎茶中黄芩苷含量测定的高效液相色谱法。方法色谱柱为ODS柱（4.6mm×250mm,5μm）,流动相为甲醇-水-磷酸（30∶70∶1）,流速为1.0mL/min,检测波长为280nm,进样10μL。结果黄芩苷在0.1988~1.1928μg范围内线性关系良好,r=0.9997,平均回收率为97.4%,RSD=1.1%。结论本方法稳定可行,结果准确可靠,可作为咽炎茶质量标准中黄芩苷的含量测定方法。
Objective To estabilsh HPLC method for determination of baicalin in Yanyan tea. Methods The chromatography column was the ODS column （4.6 mm×250 mm, 5 μm）, the mobile phase was methanol-water- Phosphoric Acid （30∶70∶1）. The flow rate was 1.0 mL/min and the detection wavelength was 280 nm, sample introduction was 10 μL. Results The calibration curve of baicalin was in good linearity over the range of 0.198 8 - 1.192 8 μg, r = 0.999 7, and the average recovery rate was 97.4% with an RSD of 1.1%. Conclusion The method is simple, reliable, accurate and can be applied to the quality control of the Yanyan tea.
出处
《中国当代医药》
2012年第10期77-78,共2页
China Modern Medicine
