摘要
建立了废水中联苯菌胺和三氟硝基联苯的高效液相色谱分析方法。采用Kromasil-C18(150 mm×4.6mm,5μm)色谱柱,流速1 mL/min,柱温30℃,进样体积10μL,流动相为V(乙腈)∶V(0.1%磷酸溶液)=60∶40,测定波长205 nm。结果线性范围为:0.05～10 mg/L,联苯菌胺的平均加标回收率为104.1%,相对标准偏差为1.65%。三氟硝基联苯的平均加标回收率为106.8%,相对标准偏差为1.37%。
The HPLC analysis method was established for the determination of biphenyl bacteria amine and 2-nitro-3′,4′, 5′-Trifluorobiphenyl in wastewater, with a Kromasil-C18 column （150 mm ×4.6mm,5μmm）, under the flow rate of 1.0 ml/ min, column temperature of 30 ℃, injection volume of 10p, L, mobile phase V （acetonitrile）/V （0.1% aqueous phosphoric acid） ratio of 60/40, and detection wavelength of 205nm. From the results, the method showed us a good linearity from 0.05 to 10 mg/L. The average recovery of standard additions of biphenyl bacteria amine and 2-nitro-3′,4′,5′-Trifluorobiphenyl were 104.1% and 106.8% respectively, and the relative standard deviations were 1.65% and 1.37%.
出处
《环境科技》
2012年第2期48-50,共3页
Environmental Science and Technology
关键词
联苯菌胺
三氟硝基联苯
高效液相色谱法
Biphenyl bacteria amine
2-nitro-3′,4′,5′-Trifluorobiphenyl
High performance liquid chromatography
