冠心Ⅱ号复方及单体组合对大鼠急性心肌梗死的影响

Effects of Compound Prescription and Monomer Combinations of Guanxin Ⅱ(GXⅡ)Prescription on Experimental Myocardial Infarct in Rats

目的:观察冠心Ⅱ号复方及单体组合对急性心肌梗死大鼠心肌酶及TTC染色心梗面积的影响,探讨冠心Ⅱ号可能药效机制。方法:通过结扎冠状动脉左前降支建立心梗大鼠模型,将模型大鼠分为模型对照组、冠心Ⅱ号复方组,冠心Ⅱ号单体组,并设假手术组和空白对照组,给药后采血测定血清CK、CK-MB、LDH水平,并取心脏行TTC染色测定心肌梗死面积。结果:与模型对照组比较,两用药组的心梗面积显著下降(P<0.01或P<0.05),但两组间无差异;心肌酶CK、CK-MB表达单体组低于复方组(P<0.05),LDH水平两组相当。结论:冠心Ⅱ号单体组在改善心梗面积方面与复方组相当的同时,表现出了优于复方组的降低心肌酶的趋势。因而进一步验证了吸收入体内的原形成分才有可能是药效成分的假说。

Objective：To compare effects of the compound prescription and monomer combinations of Guanxin Ⅱ（GXⅡ）on acute myocardial infarction in rat,and investigate GXⅡ possible pharmacodynamic action.Methods： Acute myocardial infarct（AMI）model was established by coronary artery ligation,and then the model rats were divided into model group,compound prescription of GXⅡ group, monomer combinations of GXⅡ group,and set up sham operation group,blank group.Blood was collected to determine the levels of creatine kinase（CK）,creatine kinase isoenzymes（CK-MB）and lactate dehydrogenase（LDH）.The heart of rats was cut to detect the infarcted area by TTC stain.Results： Compared to the model group,both the compound prescription of GXⅡ group and the monomer combinations of GXⅡ group significantly reduced infarct size,and blood LDH level（P0.05 or P0.01）,and they had the similar efficacy.The monomer combinations of GXⅡ group were lower than the compound prescription of GXⅡ group in Blood CK and CK-MB level（P0.05）.Conclusion： Myocardial enzyme in the monomer combinations of GXⅡ group is decreasd more than the compound prescription of GXⅡ group,while there is no obvious difference between the infarct size of two groups.It is also verified that the hypothesis of the prototype components absorbed into the body is possible efficacy components.