黄瓜矮化突变体膨胀素Cs-EXPA2基因的克隆与表达分析

Cloning and Expression Analysis of Expansion Cs-EXPA2 Gene in Dwarf Cucumber

采用RT-PCR的方法克隆了黄瓜矮化突变体中膨胀素Cs-EXPA2基因,并对其苗期88h内生长过程中mRNA水平表达量的差异进行了研究,探讨膨胀素基因对矮化的影响与作用。结果显示,Cs-EXPA2在黄瓜矮化突变体的下胚轴、根、子叶中均有不同程度的表达,利用Real-timePCR分析得出Cs-EXPA2的表达量随时空变化,Cs-EXPA2在下胚轴中的表达模式为"低-高-低",在根中的表达模式为"高-低-高"。

In this study,we cloned an expansion gene Cs-EXPA2 from dwarf cucumber（Cucumis sativus L.）D0462 by RT-PCR.We quantitatively detected its expression in hypocotyls,roots and cotyledons within 88 h during cucumber seedling stage by Real-time PCR.The result shows that the level of its expression may be related to the state of cell growth and division in these sites,and the expression level shows obvious differences at different growth stages.The express quantity of Cs-EXPA2 changes along with temporal and spatial variation.The expression pattern of Cs-EXPA2 in hypocotyls is ＇low-high-low＇,and the expression pattern in roots is＇high-low-high＇.