嫁接对西瓜根际微生物种群数量及群落结构的影响

Effects of Grafting on Rhizosphere Microorganisms Quantity and Community Structure of Watermelon

分别采用微生物培养的方法、16SrDNA PCR-RFLP方法和phlD基因PCR-RFLP方法研究了嫁接对西瓜根际微生物种群数量、细菌群落结构及拮抗菌(2,4-DAPG产生菌)群落结构的影响。结果表明:①嫁接西瓜根际细菌和真菌数量有所提高,而放线菌数量则有所减少。②嫁接西瓜与自根西瓜具有1个相同的主要根际细菌基因型;不同砧木嫁接的西瓜与自根西瓜也分别具有不同基因型的细菌。③嫁接西瓜具有与自根西瓜不同的主要根际拮抗菌基因型。

The changes of microorganisms population,bacterial community structure and 2,4-DAPG producers community structure in rhizosphere soil of grafted watermelon〔Citrullus lanatus（Thunb.） Matsum.et Nakai〕plants were studied using the methods of microorganism culture,16S rDNA PCR-RFLP and phl D gene PCR-RFLP,respectively.The results showed that the bacterial and fungal population in rhizosphere soil of grafted watermelon was increased and that of the actinomyces population was decreased;the grafted watermelon had one similar main 16S rDNA genotype in rhizosphere soil with the non-grafted watermelon,although the grafted watermelon with different stock had their unique 16S rDNA genotypes different from non-grafted watermelon;the grafted watermelon had different main 2,4-DAPG producers phl D genotypes from non-grafted watermelon.