二甲氧乙二酰甘氨酸对长期低氧状态下成骨细胞结构及功能改变的调控研究

Regulation of DMOG on the differentiation and mineralization of osteoblastic cells under long-term hypoxia

目的:通过二甲氧乙二酰甘氨酸预处理激活低氧诱导因子-1,探讨低氧状态下成骨细胞矿化及超微结构影响的调控作用。方法:获取大鼠颌面部成骨细胞,分别在低氧含量(2%O2)、常规氧含量(21%O2)及低氧含量(2%O2)+二甲氧乙二酰甘氨酸条件下进行培养,在不同培养时间分别检测成骨细胞表达的矿化、碱性磷酸酶的表达及细胞超微结构的改变。结果:加入二甲氧乙二酰甘氨酸后细胞在长期低氧状态下分泌碱性磷酸酶的能力以及钙结节形成能力无明显降低。讨论:二甲氧乙二酰甘氨酸可促进低氧状态下成骨细胞低氧诱导因子-1的高表达,有利于低氧状态下成骨细胞的结构与功能恢复,可加快成骨细胞骨形成能力。

Objective：To observe the regulatory effect of DMOG on the differentiation and mineralization in osteoblastic cells with the environment of hypoxia.Methods：Osteoblastic cells were derived from maxillofacial region of rat.The cells were cultured in three different conditions：hypoxia group（2%O2）,normal group（21%O2）and hypoxia（2%O2）＋ DMOG group.In the different time points after culture,the alkalinephosphatase（ALP） bioactivity and bone nodule formation were detected.Furthermore,the cell ultra-structure was observed by transmission electron microscope（TEM）.Results：Added with DMOG,the ALP bioactivity and the ability of bone nodule formation returned to normal.The number of mitochondria and rough endoplasmic reticulum increased in osteoblastic cells.Conclusion：Hypoxia inducible factor-1directly activated with DMOG have an ability to return the structure and function of osteoblastic cells under long-term hypoxia,which indicated that it was helpful to enhance the ability of osteogenesis.