高糖对原代培养人肾小球系膜细胞表达MMP-9、TIMP-1的影响

Effects of high glucose on the expression of MMP-9 and TIMP-1 in primary cultured human mesangial cells

目的了解高糖对原代培养的人肾小球系膜细胞表达MMP-9、TIMP-1和MMP-9/TIMP-1的影响,进一步探讨糖尿病肾病的发病机制。方法取自愿水囊引产的胎儿肾,解剖取肾皮质剪碎,应用肾皮质组织块法合优生选择法对人肾小球系膜细胞进行原代培养。ELISA方法检测MMP-9、TIMP-1的表达变化。结果与正常组相比较,高糖组MMP-9在24h、48h、72h均显著降低(P<0.01),TIMP-1在24h、72h可显著升高(P<0.05),48h表达降低;MMP-9/TIMP-1的比值在24h、48h、72h均显著降低(P<0.01)。结论高糖能够降低MMP-9/TIMP-1,与肾小球系膜细胞细胞外基质降解能力降低密切相关。

Objective To observe the effect of high glucose on the expression of MMP-9,TIMP-1 and the ratio of MMP-9/TIMP-1 in primary cultured human mesangial cells,and to elucidate their possible roles in diabetic nephropathy.Methods Kidneys isolated from voluntary induced of labor with water bag were cut into pieces,and human mesangial cells were cultured with the method of renal cortical tissue combined with eugenic selection.Then ELISA was used to measure the expression of MMP-9 and TIMP-1.Results Compared with that of the normal group,the expression of MMP-9 in the high glucose group was significantly lower at 24h,48h and 72h（P0.01）;TIMP-1was higher at 24h and 72h and lower at 48h（P 0.05）;but the ratio of MMP-9/TIMP-1 was decreased constantly at 24h,48h and 72h（P0.01）.Conclusion High glucose decreases the ratio of MMP-9/TIMP-1 in mesangial cells in vitro,which may contribute to the inhibition of ECM degradation in glomerulus.