食源性大肠杆菌的PCR检测

PCR Detection of Food-borne E.coli

为了将PCR技术更好地应用于食品安全检测,利用多重PCR对大肠杆菌uidA基因和7种毒理基因进行扩增,并对人为污染大肠杆菌的水和牛奶进行普通和实时定量PCR限度检测。结果发现,2组多重PCR反应即可完成uidA和7种毒理基因的检测;普通PCR和实时定量PCR检测限度相同,对人为污染水检测限度为2.8×102 cfu/mL,对人为污染牛奶的检测限度为2.8×104 cfu/mL。

In order to apply PCR technology in the food safety,a multiple PCR protocol was developed to detect pathogenic E.coli in food.Primers for uidA and seven virulence genes were designed,and the detection limit of E.coli were determined in artificially contaminated water and milk using general PCR and real-time PCR.It was demonstrated that two multiple PCRs can identify seven virulence gene and uidA gene for pathogenic E.coli.The detection limits of general PCR and real-time PCR test were the same： for artificially contaminated water,the detection limit was 2.8 × 102 cfu/mL;for contaminated milk,the detection limit was 2.8 × 104 cfu/mL.