酵母细胞表面展示技术

Recent Advances in Yeast Cell-Surface Display Technology

酵母细胞表达体系具备较为完善的蛋白质翻译后修饰和分泌的机制。以酵母为基础的细胞表面展示技术是一项新兴的真核蛋白展示技术,已成功应用于蛋白质识别、蛋白质的固定化和定向进化研究,成为了蛋白质工程研究的重要工具。根据与酵母细胞壁的外源目的蛋白融合部位的不同,酿酒酵母表面展示系统主要分为凝集素系统和絮凝素系统2大系统。将该技术引入动物营养研究领域,有望通过诱导宿主产生原虫或甲烷菌的抗体,对瘤胃微生态进行调控,从而以减少甲烷排放;生产高活性的全细胞催化剂,以提高反刍动物和单胃动物对纤维物质的利用效率。为此,本文就该技术的原理、特点和应用领域进行了综述。

Yeast cell has a perfect system for protein post-translational modification and secretion.The cell surface display technology based on yeast is a novel eukaryotic protein display technology.It has been successfully used for protein recognition,immobilization and remediation,and becomes an important tool for the study of protein engineering.Yeast cell surface display technology is divided into two systems,which are agglutinin and flocculin,according to the different insertion sites of the yeast cell wall for target heterologous proteins.If introduced to animal nutrition research,this technology will be a promising strategy to reduce the methane production by the regulation on ruminal microbial ecology,which is inducing of production of antibodies for protozoa and methanogen in host;highly active whole cell biocatalysts will be produced by increasing fiber utilization in ruminant and monogastric animal.Therefore,mechanism,characteristic and application of the technology are reviewed in this paper.