高水平维生素E对幼建鲤生长性能和消化吸收功能的影响

High Levels of Dietary Vitamin E Affect Growth Performance,Digestive and Absorptive Function of Juvenile Jian Carp(Cyprinus carpio var. Jian)

本试验旨在研究饲料中高水平维生素E对幼建鲤生长性能和消化吸收功能的影响。选用1 200尾平均体重(9.46±0.03)g的幼建鲤,随机分为8组,每组3个重复,每重复50尾鱼,分别饲喂维生素E水平为7、107、757、1 007、1 257、1 507、1 757和2 007mg/kg的试验饲料,试验期60d。结果表明,幼建鲤的增重,摄食量,蛋白质、脂肪和灰分沉积率,肝胰脏和肠道重量及蛋白质含量,以及肠道胰蛋白酶、糜蛋白酶、脂肪酶、碱性磷酸酶和Na+,K+-ATP酶活力均随维生素E含量的增加呈先增加后降低趋势,并在饲料中维生素E含量为1 257mg/kg时达到最大值;而饲料系数则呈相反的变化趋势。与NRC推荐量组(饲料维生素E含量107mg/kg)相比,维生素E含量为1 257mg/kg组幼建鲤前、中和后肠皱襞高度分别提高了10.85%(P<0.05)、7.48%(P>0.05)和6.24%(P<0.05)。结果提示,适宜高水平的维生素E能显著促进幼建鲤生长和消化器官生长发育,并增强幼建鲤的消化吸收能力。以增重为标识,根据二次曲线法确定幼建鲤饲料中维生素E最适水平为1 106mg/kg。

The experiment was aimed to investigate the effects of high levels of dietary vitamin E on growth performance,digestive and absorptive function of juvenile Jian carp（Cyprinus carpio var.Jian）.In a 60-day feeding trial,a total of 1 200 juvenile Jian carp with an average body weight of（9.46±0.03） g were randomly divided into 8 groups with 3 replicates per group and 50 fish per replicate.The fish in the 8 groups were fed with experimental diets containing graded levels of vitamin E： 7,107,757,1 007,1 257,1 507,1 757 and 2 007 mg/kg,respectively.The results showed as follows： with vitamin E level increasing,the weight gain,feed intake,protein production value,lipid production value,ash production value,hepatopancreas weight,hepatopancreas protein content,intestine weight,intestine protein content,and the activities of trypsin,chymotrypsin,lipase,alkaline phosphatase and Na＋,K＋-ATPase in intestinal tract of juvenile Jian carp were firstly increased and then decreased,and the highest values of above indices were found in the group with the diet containing 1 257 mg/kg vitamin E,while the feed conversion ratio showed an opposite trend.Compared with the NRC recommendation requirement group（the group with the diet containing 107 mg/kg vitamin E）,the folds height in foregut,midgut and hindgut of juvenile Jian carp in the group with the diet containing 1 257 mg/kg vitamin E was increased by 10.85%（P0.05）,7.48%（P0.05） and 6.24%（P0.05）,respectively.It is concluded that suitable high level of vitamin E can promote the growth,improve the growth and development of digestive organs and enhance the digestive and absorptive function of juvenile Jian carp.Based on the quadratic regression analysis of weight gain,the dietary vitamin E requirement of juvenile Jian carp is estimated to be 1 106 mg/kg.