参苓白术散对伊立替康化疗后大鼠迟发性腹泻的作用研究

Effect of Shenlingbaizhu San on Delayed-onset Diarrhea Caused by Irinotecan in Rats

目的:研究参苓白术散对模型大鼠伊立替康(Irinotecun,CPT-11)所致迟发性腹泻的预防作用。方法:健康雄性SD大鼠40只,随机分为正常对照组,腹泻模型组,中药低剂量组+腹泻模型组,中药中剂量组+腹泻模型组,中药高剂量组+腹泻模型组。尾静脉注射CPT-11 150mg/kg·d,连续两天,复制迟发性腹泻模型;中药十腹泻模型组于注射CPT-11前3天开始,每天一次用参苓白术散灌胃,共用10天;对各组大鼠腹泻情况进行评分;光镜下对大鼠肠黏膜损伤程度进行分级;ELISA法检测大鼠血清中IL-6,TNF-a,IL-10的水平。结果:中药各剂量组+腹泻模型组大鼠迟发性腹泻发生率均低于腹泻模型组(P<0.05);光镜下中药各剂量组+腹泻模型组大鼠肠黏膜损伤分级亦较模型组轻微(P<0.05);中药各剂量组+腹泻模型组大鼠的IL-6,TNF-a水平均比模型组低(P<0.05),而IL-10水平比模型组高(P<0.05)。结论:参苓白术散可调节大鼠免疫功能,抑制炎症反应,预防迟发性腹泻的发生。

Objecrive：To study the effect of Shenlingbaizhu San on delayed-onset diarrhea caused by Irinotecan in rats models. Methods：40 healthy male SD rats were randomly divided into normal control group,diarrhea model group,low dose ＋ diarrhea model group,rniddle dose group ＋ diarrhea model group,high dose group ＋ diarrhea model group.A model of delayed-onset diarrhea was established through injection with 150mg/kg body weigh/day CPT-11 for consecutive days into the tail vein.Herb groups were treated with Shenlingbaizhu San three days before CPT-11 injected and kept for 9 days.Scoring of delayed-onset diarrhea.histological studies of intestinal tissues under high power microscope.Elisa measuring the serum levels of IL-6,TNF-αand IL-10.Results：Scores of diarrhea in each herb dose ＋ diarrhea model group were significantly lower than those of diarrhea model group（P0.05）：the severity of intestinal injury in each herb dose ＋ diarrhea model group was profoundly reduced,compared to diarrhea model group（P0.05）;Serum levels of IL-6, TNF-αin each herb dose ＋ diarrhea model group were much lower than those of model group（P0.05）,while IL-10 levels much higher than that of model group（P0.05）.Conclusion：Shenlingbaizhu San can adjust immune function,inhibit the inflammatory response and prevent the occurrence of delayed diarrhea in rats.