Hhcy大鼠阴茎海绵体内cAMP,cGMP含量及研究

The Study about cAMP and cGMP in the Penile Corpus Cavernous of Hyper Homocysteine Rats

目的:探讨高同型半胱氨酸血症大鼠阴茎海绵体组织内cAMP,cGMP含量的变化并研究其对阴茎勃起功能影响。方法:取40只成年雄性大鼠,随机分成两组,分为正常对照组和Hhcy组。Hhcy组给予3%高蛋氨酸饲料喂养,正常组给予普通饲料喂养,饲养四周后后分别注射阿扑吗啡进行大鼠阴茎勃起功能实验,抽取血清检测Hhcy含量,麻醉后取阴茎海绵体测量cAMP,cGMP含量。结果:Hhcy组血清中同型半胱氨酸含量显著高于正常组,大鼠阴茎组织中cAMP,cGMP含量显著低于正常组。结论:高同型半胱氨酸血症大鼠阴茎海绵体中cAMP,cGMP含量降低。高同型半胱氨酸血症是阴茎勃起功能障碍的危险因素。

Objective：To detect the levels of camp,cgmp in the penile corpus cavernous of adult male Wistar rats with high homocysteine and to explore the relationship of camp and cgmp with erectile dysfunction.Methods：FoTty wistar rats were divided into a control and an Hhcy group.The control group were fed on normal diet and Hhcy group were fed diet with 3%methionine respectively.Four weeks later,the Wistar rats were detected by injecting apomorphine,the levels of camp and cgmp in the penile corpus cavenous were detect and that of serum homocysteine by the cycle enzyme method.Results：Compared with the control group,the levels of camp and cgmp in the penile corpus cavernous of the Hhcy group were significantly lower,while homocysteine was significantly higher.Conclusion：The levels of camp and cgmp in the penile corpus cavernous in Wistar rats with high homocysteine is lower.Hhcy is an significantly risk factor of erectile dysfunction.