MRP及LRP在人恶性黑色素瘤转移性淋巴结组织中的表达及临床意义研究

The expression of MRP and LRP in metastatic lymph node tissues of malignant melanoma

目的 研究多药耐药相关蛋白 (MRP)肺耐药蛋白 (LRP)在人恶性黑色素瘤转移淋巴结组织中的表达及其与以顺铂、氮唏咪胺为主的联合化疗 (PDBV)疗效的关系。方法 免疫组织化学技术对石蜡包埋组织标本进行检测。结果 2 6例晚期恶性黑色素瘤患者转移性淋巴结组织中MRP、LRP表达阳性检出率为 3 8.5 % ( 10 / 2 6)和 69.2 % ( 18/ 2 6)。MRP表达与PDBV化疗疗效无显著相关 (P >0 .0 5 ) ,而LRP阳性表达者化疗有效率明显低于阴性者 (P <0 .0 5 )。结论 MRP、LRP在人恶性黑色素瘤转移性淋巴结组织中有一定程度表达 ,检测LRP表达对临床用药的指导可能有意义。

? Objective To investigate the expression of multidrug resistance associated protein (MRP) and lung resistance protein (LRP) in metastatic lymph nodes tissues of malignant melanoma and to determine whether it was able to predict the response to chemotherapy with PDBV(DDP+DTIC+BCNU+VCR) regimen.Methods 26 Paraffin embeded metastatic lymph nodes of malignant melanoma were examined by using immunohistochemistry method.Results 10(38.5%) and 18(69.2%) of 26 metastatic lymph nodes examined showed positive immunostaining for MRP and LRP respectively.LRP Possitive patients had poor response to chemotherapy than LRP negative patients.No association was found between MRP expression and the response to chemotherapy.Conclusions Positive LRP immunostaining in metastatic lymph nodes of malignant melanoma appears to be an indicator of poor response to chemotherapy with PDBV,it may be helpful to choose anticancer drugs in chemotherapy of malignant melanoma. 〔