A和B亚群禽白血病病毒gp85基因的表达及其抗血清的亚群特异性比较

EXPRESSION OF GP85 GENE OF AVIAN LEUKOSIS VIRUSE SUBGROUP A/B AND COMPARISON OF ANTIGENIC RELATEDNESS OF THE TWO ANTI-GP85 SERUM

为制备快速鉴定禽白血病病毒A和B亚群(Avian leukosis virus subgroup A/B,ALV-A/B)的特异性诊断试剂,并鉴定分析ALV-A SDAU09C1株和ALV-B SDAU09C2株抗血清的亚群特异性,将两株病毒的gp85基因进行克隆、原核表达后,免疫家兔分别制备抗ALV-A和ALV-B的血清。血清交叉中和反应显示,SDAU09C1株和SDAU09C2株间的抗原同源性相关系数r=0.366,属于不同亚群;间接免疫荧光表明,两种抗血清均与ALV-A和ALV-B反应,但与ALV-J无反应。结果表明本试验制备的重组蛋白具有较好的反应原性,制备的二种兔抗血清可快速识别ALV-A/B与ALV-J。

In order to get a rapid specific diagnostic reagent for Avian leukosis virus subgroup A/B（ALV-A/B） detection and compare the antigenic relatedness of anti-gp85 serum of ALV-A SDAU09C1 strain and ALV-B SDAU09C2 strain,their gp85 genes were cloned.After prokaryotic expression,two rabbits were immunized by the recombinant fusion protein respectively and the serum were collected.The serological relatedness of anti-SDAU09C1 strain and anti-SDAU09C2 strain was determined using a virus-neutralization（VN） test.Relatedness（R） value between the two serums,determined by cross VN,was 36.6%,which revealed that the two strains did not belong to the same subgroup.By indirect immunofluorescence assay,anti-gp85 serums produced were reactive with ALV-A and ALV-B,but not ALV-J.The results indicated that proteins expressed maintained their antigenicity partially,and the two anti-gp85 serums could be used as specific reagent to detect ALV-A/B,but not ALV-J.