HBV感染者血清HBcAgSPRIA测定的临床分析

Clinical Analysis of Serum HBcAg Solid Phase RIA Result in HBV Infected Persons

目的 :研究HBcAg在HBV感染者中的阳性检出率、分布规律及与其他乙肝标志物的关系 ,探讨其在反映HBV复制及传染性和观察疗效方面的临床价值。方法 :采用SPRIA对461例HBV感染者进行血清乙肝六项指标测定 ,按其不同阳性结果分9种模式对比分析。结果 :HBV感染者中HBcAg阳性总检出率达50.75 % ,而未感染者及69例抗 -HBs单项阳性者中无阳性检出。结论 :SPRIA测定血清HBcAg在HBV感染者中的阳性检出具有特异性 ,并可作为疑有抗 -HBe阳性“逆转”为HBeAg阳性者的筛选检测 ,对判断HBV复制程度、病程、疗效及预后评估均有临床价值。

Objective To suudy the HBcAg positive rate, distribution pattern in HBV infected persons and its correlation to other hepatitis B marker, also to evaluate its clinical value in reflecting HBV replication, infectivity and therapeutic effect. Methods Six serum hepatitis B markers (anti-HBc, anti-HBe, HBsAg, HBeAg, anti-HBs) in 461 cases HBV infected persons were detected by SP-radioimmunoassay (SPRIA) and nine combinations of various positive and negative markers were obtained for contrast analysis. Results HBcAg positive rate was 50.75% in HBV infected persons with none positive in 90 healthy controls and 69 patients with single anti-HBs positive only SP-RIA. Conclusions Serum HBcAg SP-RIA positive in HBV infected persons is of very high specificity and the assay could be useful in those HBeAg positives possibly reversed from anti-HBe positives to determine their present HBV replication activity, infectivity as well as to assess the therapeutic effect and prognosis.