miR-221表达与K562/A02细胞耐药关系的探讨

The study on the relationship between the expression level of miR-221 and K562/A02 drug resistance

目的观察miR-221表达与K562/A02细胞耐药的关系。方法常规培养K562/A02细胞株作为对照组,选择通过反义寡核苷酸特异性抑制K562/A02细胞中miR-221表达细胞株作为实验组,应用实时荧光定量RT-PCR方法检测两组细胞中miR-221表达情况。采用MTT法比较两组细胞对化疗药物的敏感性。结果荧光定量RT-PCR结果显示,实验组细胞中miR-221表达较对照组明显降低,(P<0.05),两组比较差异倍数均值为-8.43±2.18。阿霉素对实验组细胞的IC50为0.0375mg·L-1,阿霉素对对照组细胞的IC50为4.32mg·L-1,后者的耐药倍数是实验组的115倍。结论反义寡核苷酸能够抑制K562/A02细胞中miR-221的表达,这种抑制作用能够逆转K562/A02细胞对阿霉素的耐药性。miR-221表达与K562/A02细胞耐药性密切相关。

Objective To explore the relationship between the expression level of miR-221 and drug resistance of K562/A02. Method K562/A02 was cultured as control group. The experiment group K562/A02 was treated by antisense oligonucleotide to inhibit the expression of miR-221. The realtime- PCR was used to detect the miR-221 expression level and MTT was used to detect the drug sensitivity of K562/A02 of both groups. Results The expression level of miR-221 of experiment group was significant lower than that of control group （P 0.05）. The mean different times of the two groups was -8.43±2.18. The IC50 of experiment and control group to adriamycin were 0.0375 mg·L-1 and 4.32 mg·L-1 respectively. The drug resistance times was 115 fold. Conclusion The expression of miR-221 in K562/A02 can be inhibited by antisense oligonucleotide, which may results in the reversal of drug resistance. The expression level of miR-221 is correlative to the drug resistance of K562/A02.