塞来昔布对膀胱癌细胞T24增殖的影响

Effect of Celecoxib on proliferation of bladder cancer T24 cell

目的观察塞来昔布对人膀胱癌细胞T24增殖的影响,并探讨其可能的作用机制。方法不同浓度塞来昔布(25、50、100和200μmoL·L-1)分别作用于人膀胱癌细胞T24不同时间(24h、48h和72h),MTT检测T24细胞增殖抑制率,半定量RT-PCR和Western-blot检测T24细胞中COX-2表达的变化。结果塞来昔布对膀胱癌T24细胞抑制作用存在剂量、时间依赖性,200μmoL·L-1塞来昔布作用72h时抑制率达75.7±1.9%,明显高于其它各组(P<0.05或0.01);半定量RT-PCR和Western-blot方法显示塞来昔布可显著降低T24细胞中COX-2mRNA和蛋白水平(P<0.05),且呈时间依赖性。结论塞来昔布可通过减少COX-2的表达抑制人膀胱癌细胞T24的增殖,并呈时间和剂量依赖性。

Objective To investigate the inhibitory effect of celecoxib on the growth of human bladder cancer cell T24 and its possible mechanism.Method The human bladder cancer cells T24 were treated respectively with 25,50,100 and 200μmoL·L^-1 celecoxib for 24,48 and 72 h.The inhibitory rate was detected by MTT assay,COX-2 mRNA levels by semiquantitative RT-PCR,and COX-2 protein levels by Western-blot.Results Celecoxib inhibited the proliferation of T24 in a time-dependent and dose-dependent manner.The inhibitory rate of 200μmoL·L^-1 celecoxib was 75.7±1.9% when treated for 72 hours,significantly higher than that in other groups （P〈0.01 or 0.05）.Semiquantitative RT-PCR and Western blotting demonstrated that the levels of COX-2 expression decreased in a time-dependent manner in T24 bladder cancer cells after administration of celecoxib （P〈0.05）.Conclusion Celecoxib inhibited the proliferation of human bladder cancer cell T24 in a dose-dependent and time-dependent manner by decreasing the expression of COX-2.