胆囊癌组织中BRAF和Caspase-3的表达与相关性研究

The expression its implication of BRAF and Caspase-3 in gallbladder carcinoma and

目的探讨胆囊癌组织中BRAF和Caspase-3的表达水平,了解胆囊癌组织中BRAF和Caspase-3的表达的相关性。方法采用免疫组织化学法检测68例组织中BRAF和Caspase-3的蛋白表达水平(49例胆囊癌、9例胆囊腺瘤、10例胆囊炎)。结果在所检测的胆囊癌组织中,BRAF阳性表达率为65.3%(32/49),明显高于胆囊炎和胆囊腺瘤组(P<0.01);而Caspase-3在胆囊癌组织中阳性表达率为42.9%(21/49),明显低于胆囊炎和胆囊腺瘤组(P<0.01)。BRAF表达与肿瘤的细胞学分级、Nevin分期及性别年龄均无统计学意义(P>0.05)。Caspase-3表达与分化程度有关而与Nevin分期及性别年龄等无关。BRAF与Caspase-3表达呈负相关(r=-0.373,P=0.008)。结论对BRAF和Caspase-3的联合检测可能作为胆囊癌诊断、预后判断的指标。

Objective To dectect the expression of BRAF and Caspase-3 in gallbladder carcinoma and to explore their correlation with invasion. Methods The expression of BRAF and Caspase-3 were detected with immunohistochemistry in 68 cases （include 49 cases of gallbladder carcinoma and 9 cases of gallbladder aderloma and 10 cases of cholecystitis）. Resuits In response to the detected in gallbladder carcinoma tissue, the positive expression rate of BRAF was 65.3% （32/49）, the positive expression rate of BRAF was significantly higher than that of cholecystitis and gallbladder adenoma group.But, Caspase-3 in gallbladder carcinoma tissue positive expression rate of was 42.9%（21/49）, the positive expression rate of Caspase-3 was significantly lower in cholecystitis and gallbladder adenoma group.BRAF expression and tumor cytologic grading showed no relationship,while the expression of Caspase-3 was associated with differentiation. The expression of survivin cor- related inversely with Caspase-3（r = -0.373,P = 0.008）. Conclusion Therefore,the joint detection of BRAF and Caspase- 3 expression may serve as an indicator for judging diagnosis and prognosis metastatic potential of gallbladder carcinoma.