青阳参总甙对小鼠四氯化碳肝损伤的保护作用

PROTECTION OF QINGYANGSHENYLYCOSIDE ON HEPATIC INJURY INDUCED BY CARBON TETRACHLORIDE IN MICE

目的探讨青阳参总甙对四氯化碳(CCl4)所致小鼠急性肝损伤的保护作用及其机制。方法健康小鼠60只随机分为6组(各10只),对照组和模型组每天腹腔注射生理盐水,联苯双酯组小鼠灌胃给予联苯双酯150mg/(kg.d),青阳参总甙低、中、高剂量组每天分别腹腔注射青阳参总甙溶液20、40、80mg/kg,连续7d。末次给药1h后,除对照组腹腔注射花生油溶液10mL/kg外,其余各组腹腔注射含体积分数0.001的CCl4花生油溶液10mL/kg,建立急性CCl4小鼠肝损伤模型。16h后摘眼球取血,分别测定血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、白细胞介素6(IL-6)、肿瘤坏死因子-α(TNF-α)含量。处死小鼠取其肝脏测定肝组织中超氧化物歧化酶(SOD)和丙二醛(MDA)的水平,并进行肝脏病理学检查。结果与模型组比较,青阳参总甙各剂量组小鼠血清中ALT、AST、IL-6、TNF-α水平和肝组织匀浆中MDA的含量显著降低,肝组织匀浆中SOD的活性升高,差异均有显著性(F=2.766～23.952,q=2.161～9.305,P<0.05、0.01),肝组织病理损伤程度减轻。结论青阳参总甙对CCl4诱导的急性化学性肝损伤具有明显的保护作用,其机制可能与抗氧化和减少炎性细胞因子的释放有关。

Objective To explore the protective effect of Qingyangshenylycosides（QYS） on hepatic injury induced by carbon tetrachloride（CCl4） in mice and its mechanism. Methods Sixty healthy adult mice were equally randomized to six groups.The mice in control group and model group were given same volume of normal saline intraperitoneally,once a day;those in dimethyl diphenyl bicarboxylate（DDB） group were given intragastric administration of DDB,150 mg/（kg ·d）;those in low-dose-,medium-dose-and high-dose-QYS group were given intraperitoneal injection of 20,40,and 80 mg/（kg·d）,respectively,lasting for seven days.One hour after the last medication,the mice in all groups,except the control group,were intraperitoneal injection of 1 mL/L CCl4 peanut oil solution 10 mL/kg to create a hepatic-injury model in mice.Sixteen hours later,the eye balls of the mice were removed,blood taken,the mice were then killed and liver collected.Serum alanine aminotrasferase（ALT）,aspartate aminotrasferase（AST）,interleukin-6（IL-6）,tumor necrosis factor alpha（TNF-α）,the levels of superoxide dismutase（SOD）,malondialdehyde（MDA） in liver tissue were measured.A pathology examination for liver was conducted. Results As compared with the model group,the levels of serum ALT,AST,IL-6,TNF-α and levels of MDA in hepatic tissue in each QYS group were notably reduced,and the activity of SOD increased,the differences being significant（F=2.766-23.952;q=2.161-9.305;P〈0.05,0.01）,the damage of liver lightened as well. Conclusion QYS has a protective effect on acute damage of liver in mice induced by CCl4,the mechanism might be related to anti-oxidant and decrease of releasing inflammatory cytokines.