急性白血病病人骨髓细胞BAALC基因表达及意义

EXPRESSION AND CLINICAL SIGNIFICANCE OF BAALC GENE IN PATIENTS WITH ACUTE LEUKEMIA

目的通过检测脑和急性白血病胞质(BAALC)mRNA在急性白血病(AL)病人表达,探讨其与AL病情进展及预后关系。方法应用逆转录聚合酶链反应技术,检测20例急性髓系白血病(AML)、8例急性淋巴细胞白血病(ALL)及11例特发性血小板减少性紫癜(ITP)、缺铁性贫血(IDA)等非恶性血液病病人(对照组)骨髓单个核细胞BAALC mRNA表达水平。结果初治组AL病人BAALC mRNA表达水平较对照组显著升高(F=8.59,q=5.62,P<0.05);经治疗获完全缓解后,与初治组比较,其表达水平显著下降,两组比较差异有显著性(q=3.99,P<0.05)。BAALC mRNA表达水平与AL病人外周血白细胞计数及骨髓原始细胞比例无明显相关性(P>0.05)。结论 BAALC mRNA表达与AL疾病进展及预后密切相关,可作为AL预后判断的有意义指标。

Objective To detect the expression of brain and acute leukemia cytoplasmic（BAALC） gene in patients with acute leukemia（AL）,and explore the relationship between the expression and the outcome of AL. Methods The expression of BAALC mRNA was detected,by reverse transcription-PCR,in mononuclear cells of 20 patients with AML,eight with ALL and 11 with idiopathic thrombocytopenic purpura（ITP）,iron deficiency anemia（IDA） and other non-malignant hematological diseases. Results In newly diagnosed patients with AL,the expression of BAALC mRNA was obviously higher than that in the normal controls（F=8.59,q=5.62,P〈0.05）.When the patients achieved complete remission,the expression of BAALC mRNA was markedly lower than that of the newly diagnosed group,the difference was statistically significant（q=3.99,P〈0.05）.The expression of BAALC mRNA in patients with AL was not correlated with peripheral blood cell counts and the proportion of bone marrow blast cells. Conclusion The expression of BAALC mRNA is closely associated with the progression and prognosis of AL,which can be used as a significant parameter to predict the prognosis of AL.