Ph阴性骨髓增殖性肿瘤病人JAK2基因表达及意义

THE EXPRESSION AND ITS CLINICAL SIGNIFICANCE OF JAK2 IN Ph-NEGATIVE MYELOPROLIFERATIVE NEOPLASMS

目的探讨Ph阴性的骨髓增殖性肿瘤(MPN)病人骨髓细胞中Janus激酶2(JAK2)基因的表达及其临床意义。方法用常规染色体分析法检测MPN病人的染色体,选择Ph阴性病人16例作为观察组,继发性红细胞增多症和继发性血小板增多症病人12例为病例对照组,以健康人5例为正常对照组,应用半定量RT-PCR方法分别检测各组JAK2mRNA的水平。结果 Ph阴性MPN病人JAK2mRNA的平均表达水平为0.63±0.35,病例对照组为0.62±0.43,正常对照组为0.60±0.25,各组间相比较差异无统计学意义(P>0.05)。结论JAK2mRNA的检测不能单独作为MPN与继发性红细胞增多症及继发性血小板增多症的鉴别诊断指标。

Objective To investigate the expression of JAK2 and its clinical significance in patients with Ph-negative myeloproliferative neoplasms（MPN）. Methods A conventional karyotype analysis was used to detect the chromosomes in patients with MPN,those 16 with Ph-negative were enrolled as observation group,and five healthy subjects served as normal control.JAK2 mRNA levels were detected in each group by employing semi-quantitative reverse transcriptase polymerase chain reaction（RT-PCR） technique. Results The average levels of JAK2 mRNA in Ph-negative patients,case controls and healthy controls were 0.63±0.35,0.62±0.43 and 0.60±0.25,respectively.The differences of JAK2 mRNA levels between MPN patients and case controls and healthy controls were not significant（P〈0.05）. Conclusion The JAK2 mRNA level can not be used alone as a diagnosis index in the differential diagnosis among MPN,secondary erythrocytosis,and secondary thrombocythemia.