半胱氨酰白三烯受体1参与鱼藤酮诱导PC12细胞损伤的调节

Cysteinyl leukotriene receptor 1 is involved in rotenone-induced injury of PC12 cells

目的:观察半胱氨酰白三烯受体1(CysLT1受体)与鱼藤酮诱导PC12细胞损伤的关系。方法:鱼藤酮及CysLT1受体拮抗剂孟鲁司特处理PC12细胞后,以MTT方法检测PC12细胞活性变化;以Western blotting检测鱼藤酮处理前后,PC12细胞CysLT1受体的表达变化;不同浓度鱼藤酮处理24 h及3μmol/L鱼藤酮处理不同时间,以免疫细胞化学方法观察CysLT1受体分布变化。结果:鱼藤酮(0.3～30μmol/L)可诱导PC12细胞损伤,孟鲁司特(1、5μmol/L)可显著减轻鱼藤酮诱导的细胞损伤。1～10μmol/L鱼藤酮作用PC12细胞24 h及3μmol/L鱼藤酮处理3 h和24 h,均可诱导CysLT1受体蛋白水平表达升高。鱼藤酮能浓度和时间依赖性引起CysLT1受体从细胞核移位到细胞浆。结论:CysLT1受体参与鱼藤酮诱导的PC12细胞损伤。

Objective： To investigate whether cysteinyl leukotriene receptor 1（CysLT1 receptor） is involved in rotenone-induced injury of PC12 cells.Methods： After 24 h treatment with rotenone or with rotenone and the CysLT1 receptor antagonist montelukast,PC12 cell viability was determined by the colorimetric MTT reduction assay.After PC12 cells were treated with various concentrations of rotenone for 24 h or with 3 μmol/L rotenone for various durations,the expression of CysLT1 receptor was determined by Western blotting,and its intracellular distribution was detected by immunocytochemistry.Results： Rotenone（0.3-30 μmol/L） induced PC12 cell injury;this injury was significantly attenuated by montelukast at 1 and 5 μmol/L.The expression of CysLT1 receptor increased after rotenone treatment at 1-10 μmol/L,or at 3 μmol/L for 3 and 24 h.Rotenone caused concentration-and time-dependent translocation of CysLT1 receptor from the nucleus to the cytosol.Conclusion： Cysteinyl leukotriene receptor 1 is involved in rotenone-induced injury of PC12 cells.