p53codon72单核苷酸多态性与肝细胞癌发病风险的关系

Association of p53 Codon72 Polymorphism and Risk of Hepatocellular Carcinoma

[目的]探讨广西地区p53基因codon72单核苷酸多态性(SNP)与肝细胞癌(HCC)发病风险的关系。[方法]采用TaqMan MGB探针等位基因分型技术对985例肝癌病例和相匹配的992例非肿瘤对照的p53 codon72(Arg>Pro,rs1042522)基因型进行检测,并分析该SNP与肝癌发病风险的关系。[结果]p53 codon72多态性与肝癌发病风险之间无统计学关联(Arg/Pro:校正OR=1.15,95%CI:0.83～1.59;Pro/Pro:校正OR=1.16,95%CI:0.80～1.68;Arg/Pro+Pro/Pro:校正OR=1.15,95%CI:0.85～1.57)。按是否吸烟、饮酒、HBV和HCV感染分层分析,亦未发现p53 codon72多态性与肝癌发病风险有关。但基因—环境交互作用显示,该基因多态性与吸烟、饮酒和HBV感染存在交互作用,OR值分别为2.42(95%CI:1.47～3.97)、2.96(95%CI:1.82～4.80)和62.74(95%CI:34.39～114.46)。[结论]p53codon72的单独效应可能与肝癌易感性无关联,但该SNP与吸烟、饮酒和HBV感染存在基因—环境交互作用,增加肝癌的发病风险。

[Purpose] To explore associations between p53 single nucleotide polymorphism（SNP） codon72 and risk of hepatocellular carcinoma（HCC） in Guangxi.[Methods] A hospital-based case-control study was conducted in 985 cases with HCC and 992 cancer-free matched controls.p53 codon72 genotypes（ArgPro,rs1042522） were detected by Applied Biosystems TaqMan genotyping platform.[Results] The variant genotypes of p53 codon72 did not significantly associate with risk of HCC（Arg/Pro：adjusted OR=1.15,95%CI：0.83～1.59;Pro/Pro：adjusted OR=1.16,95%CI：0.80～1.68;Arg/Pro＋Pro/Pro：adjusted OR=1.15,95%CI：0.85～1.57）.After stratified with smoking,alcohol drinking,HBV and HCV infections,no risk increasing genotype was found.However,interactions between p53 codon72 polymorphism and smoking,alcohol drinking and HBV infection might modify the risk of HCC [smoking（adjusted OR=2.42,95%CI：1.47～3.97）,alcohol drinking（adjusted OR=2.96,95%CI：1.82～4.80） and HBV infection（adjusted OR=62.74,95%CI：34.39～114.46）].[Conclusion] p53 codon72 may not have the independent effect on the susceptibility to HCC.However,the interactions between p53 codon72 polymorphism and smoking,alcohol drinking and HBV infection might modify the risk of HCC.