氯吡格雷对人脐静脉内皮细胞全基因表达谱的影响及生物信息学分析

Impact of Clopidogrel on Gene Profile of Human Umbilical Vein Endothelial Cell Line and Bioinformatics Analysis

本研究探讨氯吡格雷对体外培养人脐静脉内皮细胞(EA.hy926)基因表达谱的影响及作用的分子机制。将10μmol/L氯吡格雷与人脐静脉内皮细胞株共培养48 h,运用Affymetrix U133 plus2.0全基因组表达芯片检测氯吡格雷对人脐静脉内皮细胞基因表达谱的影响,应用分子注释系统MAS3.0软件进行聚类分析,实时定量RT-PCR验证基因表达谱结果。结果表明:氯吡格雷作用内皮细胞48 h后,基因芯片分析筛选出差异表达大于1.5倍的基因508个,其中上调基因139个,下调基因369个,包括蛋白结合、转录因子活性、锌离子结合、DNA依赖的转录的调节、转录、RNA剪接等相关基因,RT-PCR验证结果与基因芯片结果一致。结论:氯吡格雷在基因水平通过多条通路调节内皮功能。

This study was purposed to investigate the effect of clopidogrel on gene expression profile of cultrured human umbilical vein endothelial cell（HUVEC） line（EA.hy926）,and explore its potential molecule mechanism. A Affymetrix U133 plus 2.0 oligonucleotide microarray was applied to detect the alteration of gene expression profile induced by clopidogrel in HUVEC.Real time RT-PCR was used to verify the result of selected differentially expressing genes.The results showed that total 508 genes（including 139 up-regulated and 369 down-regulated genes） were obtained with differential expression more than 1.5-fold after clopidogrel（10 μmol/L） acted on HUVEC for 48 h.Clopidogrel affec-ted the expression levels of genes involved protein binding,transcription factor activity,zinc ion binding,regulation of DNA-dependent transcription,transcription,RNA splicing and so on.It is concluded that the clopidogrel modulate function of endothelial cells by regulating sets of genes through different paHTHay.