姜黄素对肾癌786-O细胞增殖及凋亡的实验研究

Experiment research of curcumin on the proliferation and apoptosis of human renal cell carcinoma cell line 786-O

目的观察姜黄素对肾癌786-O细胞缺氧诱导因子-1α(HIF-1α)和X连锁凋亡抑制蛋白(XIAP蛋白)表达水平以及细胞凋亡的影响,研究姜黄素对肾癌细胞的生长抑制作用并探讨其分子机制,进一步揭示姜黄素对肾癌的治疗作用。方法不同浓度姜黄素作用人肾癌786-O细胞24、48、72 h后,应用四甲基偶氮唑蓝(MTT)比色法检测姜黄素对人肾癌786-O细胞的增殖抑制率;流式细胞术检测姜黄素诱导细胞的凋亡率;免疫细胞化学检测姜黄素对786-O细胞HIF-1α和XIAP表达的影响。结果姜黄素对人肾癌786-O细胞有明显的抑制作用,可引起细胞凋亡,并且存在剂量和时间依赖;不同浓度姜黄素作用细胞48 h后,HIF-1α和XIAP蛋白表达量下降。结论姜黄素通过下调HIF-1α和XIAP的表达抑制人肾癌786-O细胞的增殖,诱导人肾癌786-O细胞的凋亡。

Objective To investigate the effects of curcumin on renal cell carcinoma 786-O cells HIF-1α and XIAP protein expression level and cell apoptosis,study on the effect of curcumin on renal carcinoma cell growth inhibition and to explore the molecular mechanism of curcumin on renal cell carcinoma,and further reveals the therapeutic effect.Methods The human renal cell carcinoma 786-O cells were treated with curcumin of different concentrations for 24 h,48 h and 72 h respectively.The MTT test was used to evaluate the proliferation inhibition of curcumin on renal cell carcinoma 786-O cells.The flow cytometry were utilized to observe and detect the apoptosis of renal cell carcinoma 786-O cells induced by curcumin.The expression of HIF-1α and XIAP protein were evaluated by immunocytochemical detection method.Results The curcumin of different concentrations could significantly inhibit the proliferation and induce apoptosis of renal cell carcinoma 786-O cells,with obvious dose-dependent and time-dependent effects.With intervention of different concentrations of curcumin for 48 h,the expression of HIF-1α and XIAP protein in renal cell carcinoma 786-O cells was decreased as compared with those in the untreated group.Conclusion The proliferation inhibiting effects of curcumin on human renal cell carcinoma cell line 786-O may be related to down-regulating HIF-1α and XIAP,and promote the apoptosis.