支气管哮喘患者血清肿瘤坏死因子-α、白细胞介素-6及白细胞介素-8水平变化研究

Research on the changes of serum TNF-α,IL-6 and IL-8 levels in patients with bronchial asthma

目的观察支气管哮喘患者血清肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)及白细胞介素-8(IL-8)水平的变化并探讨其在支气管发生发展中的作用。方法采用酶联免疫吸附法(ELISA)分别检测支气管哮喘急性发作组、缓解组和正常对照组的血清TNF-α、IL-6及IL-8水平,并对其差异、关联性进行分析。结果支气管哮喘患者血清肿瘤坏死因子TNF-α、IL-6及IL-8水平显著高于对照组(P<0.05),且3种细胞因子之间均存在明显的正相关关系(P<0.05)。结论支气管哮喘患者血清TNF-α、IL-6及IL-8水平升高,受到疾病严重程度的影响,3种细胞因子均有可能参与了哮喘的炎性反应。

Objective To observe the changes of serum TNF-α,IL-6 and IL-8 levels in patients with bronchial asthma and to explore the role of TNF-α,IL-6 and IL-8 in the development of the disease.Methods The enzyme-linked immunosorbent assay（ELISA） was used to detect the levels of serum TNF-α,IL-6 and IL-8 for the patients of the bronchial asthma acute exacerbation group,the asthma remission group and the control group,and then their differences and correlation were analyzed.Results The levels of TNF-α,IL-6 and IL-8 of bronchial asthma patients were significantly higher than those of the control group（P 0.05）,and positively correlation were existed among the three cytokines significantly（P 0.05）.Conclusion The levels of serum TNF-α,IL-6 and IL-8 in patients with bronchial asthma are higher than the normal,which are impacted by the severity of the disease,and the three cytokines are likely involved in the inflammatory response of asthma.