宝霍甙元对大鼠心肌缺血再灌注损伤的保护作用

Protective Effect of Baohuoside Aglycone in Myocardial Infarction of Ischemia/reperfusion Injury in the Rat

目的观察宝藿甙元在大鼠心肌缺血再灌注损伤中的保护作用。方法用在体左冠状动脉前降支穿线结扎法制备心肌缺血再灌注模型。36只SD大鼠分为3组,假手术组12只,缺血再灌注组12只,宝藿甙元治疗组12只。三组SD大鼠均继续饲养三周,术后三天检测每组血清SOD浓度,三周后检测心肌梗死重量的比率。结果血清SOD浓度在宝藿甙元组较缺血再灌注组比较(6.54±0.41 vs.5.64±0.68,P=0.003)有明显升高且有统计学意义,宝藿甙元组与假手术组比较(6.54±0.41 vs.6.27±0.48,P=0.17)虽有增高,但无统计学意义。宝藿甙元治疗组较缺血再灌注组的心肌梗死重量比率有明显减少(9.03±0.22%vs.31.83±2.09%,P<0.01)且有统计学意义。结论宝藿甙元可以增加缺血再灌注后机体的抗氧化作用,从而起到对缺血再灌注损伤后的心肌有保护作用,可以减轻心肌坏死的程度。

Objective To observe the protective effects of Baohuoside aglycone（BA） in myocardial Infarction of ischemia/reperfusion（I/R） injury in the rat.Methods Myocardial ischemia/reperfusion injury model was induced by ligation the left descending anterior coronary artery（LAD）.Thirty-six SD rats were divided into three groups： sham group,I/R injury group and BA treated group,each contained 12 rats respectively.The serum superoxide dismutase（SOD） was tested 3 days after surgery and the rate of the infraction muscle weight was measured 21 days after surgery.Result The serum SOD in BA treated group is higher than that in I/R injury group（6.54±0.41 vs.5.64±0.68 P=0.003）.The rate of the BA treated group is lower than that in I/R injury group（9.03± 0.22% vs.31.83±2.09%,P〈0.01）.Conclusion Due to the reduction of the injury of free radicals,Baohuoside aglycone possesses cardioprotective effect in prevention of ischemia/reperfusion injury and reduce the myocardial infraction.