基质金属蛋白酶9及其启动子C-1562T基因多态性与冠心病的相关性研究

The research of matrix metalloproteinase-9 and its promoter C-1562T gene polymorphism with coronary heart diseaseThe research of matrix metalloproteinase-9 and its promoter C-1562T gene polymorphism with coronary heart disease

目的探讨基质金属蛋白酶9（MMP-9）及其启动子C-1562T基因多态性在冠心病发病中的作用及其临床意义。方法入选163例患者，冠心病组103例，其中急性冠脉综合征（ACS）组71例，稳定型心绞痛（SAP）组32例；对照组60例。采用限制性内切酶片段长度多态性（PCR—RFLP）法鉴定MMP-9启动子C-1562T基因型，所有患者均行冠脉造影检查明确冠状动脉病变情况。结果①冠心病组MMP-9启动子-1562C／T基因型频率较对照组增高，-1562T型等位基因频率增高，差异有统计学意义。②冠心病患者MMP-9浓度较对照组明显升高，差异有统计学意义。③有T型等位基因患者MMP-9浓度较无T型等位基因患者升高，差异有统计学意义。④冠心病与其危险因素logistic回归分析提示，吸烟、高血压、高脂血症、肥胖、MMP-9启动子-1562T型等位基因为冠心病的危险因素。结论MMP-9启动子C-1562T基因多态性与冠心病的发病可能相关，T型等位基因可能是冠心病患者遗传易感性基因标志之一。MMP-9启动子C-1562T型等位基因可能引起MMP-9表达增高。

Objective To investigate metalloproteinase-9 and its promoter C-1562T genetic polymorphism in the pathogenesis course of coronary heart disease （CHD） and clinical significance. Methods Selected 163 cases patients, of which CHD group were 103 eases, ACS 71 cases, SAP 32 cases, 60 eases in the control group, used polymerase chain reaction restriction fragment length polymorphism （PCR-RFLP） to identify genotype of MMP-9 promoter C-1562T. All patients underwent selective coronary angiography, to identify coronary artery disease. Results （1）Compared with control group, MMP-9 promoter-1562C/T genotype frequency and -1562T al- lele frequency increased in CHD group, there were statistically difference. （2）Compared with control group, MMP- 9 concentration increased signifieantly in CHD group, there were statistically difference. （3）MMP-9 concentration increased in T-allele patients compared with no T-allele, there were statistically difference. （4）To logistic regression analyze between CHD and its risk factors, suggested that smoking, hypertension, hyperlipidemia, obesity, MMP-9 promoter -1562T allele were risk factors for CHD. Conclusion The present findings suggest that genetic polymorphism in MMP-9 promoter C-1562T is associated with CHD, T-allele may be one of signs of genetic susceptibility genes in patients with CHD, -1562T allele may increase expression of MMP-9.